Key Technology for Seedling Culture of Gametophyte in Kelp Saccharina japonica
SUN Juan1,2, CHEN Shuxiu1,3, ZHAO Nan1,3, SHI Liang1,2, WANG Weiwei1,3, LI Xiaojie1,2, LI Xia1,2
1. Shandong Oriental Ocean Group Co., Ltd., Yantai 264003, China; 2. National Algae and Sea Cucumber Engineering Technology Research Center, Yantai 264003, China; 3. Shandong Key Laboratory of Seawater Seed Industry (Constructed), Yantai 264003, China
Abstract：In order to improve the utilization rate of gametophyte and seedling breeding efficiency in kelp Saccharina japonica, the suspension of about 100 μm of cell segments after 30—60 s of breaking up the female and male gametophytes was mixed evenly, added to a petri dish (φ=12 cm), and placed under white light, blue light, red light, and red-yellow blue light and available nitrogens of NaNO3, CO(NH2)2, NH4NO3, NH4Cl, and NH4HCO3 at concentrations of 10 mg/L. The technology of multiple seedling attachment of gametophytes was developed in the seedling stage, and the effects of double-curtain culture methods and different seedling environments on seedling growth were investigated, and the key technology of gametophyte seedlings was optimized. When the first seedlings were cultivated to the newly trans-sporophytes, the well-developed gametophytes were collected with 38 μm sieve silk and sprayed on the new seedling curtain for the second seedlings. When the seedlings of the first seedling were cultivated to 2—4 rows of juvenile sporophytes, washed the seedling curtain again, collected the sporophytes and a small amount of gametophytes, and sprayed them on the new seedling curtain for the third seedlings. The results showed that the developmental rates of gametophyte were significantly improved under blue light and urea as nitrogen source. The seedlings on the second and third attached seedling curtains were shown to grow well, with the poor length than that on first attached seedling curtain. The seedlings were required to be cultured in 48 d for stocking on the sea for the first attached seedlings, 39 d for the second ones and 33 d for the third ones, with the shortened breeding cycle. The seedlings were shorter in length in the lower layer of the double-layer curtain than those in the upper layer due to short illumination period, and the size and shape of the seedlings were affected by the environment of the breeding pool, and thus adjusting the position of the seed curtain in the breeding pool was beneficial to the uniformity of seedling sizes. The comprehensive utilization of multiple attachment techniques and double-layer curtain culture methods not only improved the utilization of gametophyte, but also improved the seedling yield per unit water surface and shortened the breeding cycle and reduce in the production cost.
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2026, Vol. 45 
