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| Effects of Different Floc Particle Sizes on Nitrification in Biofloc Technology Aquaculture System |
| CHEN Xiaoqing1, LUO Guozhi1,2,3, TAN Hongxin1,2,3, WU Huifang1, MENG Haoyan1, LI Shuang1 |
1. Research and Development Center of Aquaculture Engineering of Shanghai, Shanghai Ocean University, Shanghai 201306, China;
2. Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China;
3. National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China |
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Abstract After biofloc were cultured for 50 days, three groups of different particle size flocs were sieved through a 300 mesh sieve which named as un-sieved bioflocs, <50 μm particle size and >50 μm particle size, respectively. A total of 10 mg/L total ammonia nitrogen(TAN) and glucose were added into the three reactors with the C/N ratio of 15. The removal effect of nitrogen in the water was investigated. It was showed that there was no significant differences in the assimilation removal rate of ammonia nitrogen with different size flocs(P＞0.05).The bacterial community of bioflocs was analyzed by Illumina MiSeq sequencing analyzes. The results showed that the microbial community composition of the different floc particle size groups was similar at the levels of phylum, class and genus. The main bacteria in the process of biofloc culture belonged to 6 classes: Actinobacteria, α-Proteobacteria, β-Proteobacteria, γ-Proteobacteria, Bacilli and Sphingobacteriia. In the genus level, Rhodococcus, Microbacterium, Hydrogenophaga and Bacillus were dominant bacteria. Other bacterium genera include Paracoccus, Pseudomonas, Aquimonas, Mycobacterium, Candidatus_Alysiosphaera and some unknown ones. There was significantly higher abundance of Firmicutes (Bacillus) in the <50 m particle size group than that in the other two groups(P＜0.05), and particle size had a significant effect on the abundance of Firmicutes (Bacillus)(P＜0.05). The addition of carbon source can improve the stability and cushioning of the microbial community.
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Received: 11 March 2019
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