泰国斗鱼Dmrt1基因的部分cDNA克隆及表达分析

doi:10.16378/j.cnki.1003-1111.19238

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Abstract Doublesex and Mab-3 related transcription factor 1 (Dmrt1), a member of DMRT family, plays critical roles in sex determination, sex differentiation and gonadal development. The molecular cloning of a partial sequence of Dmrt1 was performed in the brain tissue of Siamese fighting fish Betta splendens by RACE, and Dmrt1 gene expression pattern was detected in all tissues and embryonic development of Siamese fighting fish by semi-quantitative RT-PCR and real-time quantitative RT-PCR. Siamese fighting fish had Dmrt1 consisting of 1037 nucleotides, with a 798 bp length of open reading frame (ORF), and coding 265 amino-acids. Phylogenetic analysis showed that Dmrt1 in Siamese fighting fish was more closely related to climbing perch Anabas testudineus. In addition, tissue-specific and sexual dimorphism expression profile of Dmrt1 was detected in Siamese fighting fish. In males, Dmrt1 was expressed in the testis and spleen exclusively. In females, however, Dmrt1 was highly expressed in the heart, weakly expressed in the brain, pituitary, spleen, muscle, kidney and intestine, without expression in ovary and liver. Moreover, during the period of embryonic development, Dmrt1 was predominately expressed in the fertilized eggs, and weakly from the gastrula stage to heart beating stage. The findings indicate that Dmrt1 may play an important role in male germ cell development, and reproductive regulation in spermatocyte maturation in Siamese fighting fish.

Key words： Betta splendens Dmrt1 gene cloning tissue distribution embryo development

Received: 28 October 2019

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	Articles by authors
	HUANG Yang
	LI Jinhai
	WANG Wenji
	GUO Yuwen
	LI Guangli
	CHEN Huapu

Cite this article:

HUANG Yang,LI Jinhai,WANG Wenji, et al. Molecular Cloning, Partial Sequence and Tissue Expression of Dmrt1 Gene in Siamese Fighting Fish Betta splendens[J]. Fisheries Science, 2021, 40(4): 618-623.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.19238 OR https://www.shchkx.com/EN/Y2021/V40/I4/618

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