高温与氨氮复合胁迫对凡纳滨对虾渗透调节的影响

doi:10.16378/j.cnki.1003-1111.19281

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Abstract The changes in Na⁺/K⁺-ATPase and Ca²⁺-ATPase activities in gill and the contents of free amino acid (FAA) in muscle and gill were monitored in Pacific white shrimp Litopenaeus vannamei with body weight of (6.77±0.51) g reared in 200 L fiberglass barrels at initial stocking density of 50 per barrel under two temperatures (normally 30 ℃ and acute increase from 30 ℃ to 33 ℃ within 1 h) × 4 ammonia nitrogen concentrations (0, 5, 15, and 25 mg/L) adjusted with ammonium chloride solution for 72 h to evaluate the effects of ammonia-nitrogen stress on the physiological osmoregulation of Pacific white shrimp under acute warming temperature. The results showed that the activities of Na⁺/K⁺-ATPase and Ca²⁺-ATPase were found to be significantly increased in 25 mg/L and 5 mg/L ammonia groups at 30 ℃(P<0.05), significant increase in activities of Na⁺/K⁺-ATPaes and Ca²⁺-ATPase in all ammonia groups at 33 ℃(P<0.05). In the same ammonia concentration, there were significant differences in the activities of Na⁺/K⁺-ATPase and Ca²⁺-ATPase between 30 ℃ and 33 ℃ groups, significantly lower enzyme activity in the 33 ℃ group than that in the 30 ℃ group at 25 mg/L ammonia concentration (P<0.05). In gill and muscle, the total contents of FAA were significantly lower in the 33 ℃ group than that in 30 ℃ group at ammonia concentration of 15 mg/L and 25 mg/L (P<0.05). Under ammonia stress, alanine level in muscle was increased significantly in 30 ℃ and 33 ℃ groups with increasing ammonia concentration (P<0.05), and significantly higher at 33 ℃ than at 30 ℃ group (P<0.05).The findings indicated that ammonia stress sharply affected Na⁺/K⁺-ATPase and Ca²⁺-ATPase activities as well as the contents of FAA under acute warming temperature,leading to dysfunction of the physiological osmoregulation of Pacific white shrimp.

Key words： Litopenaeus vannamei temperature ammonia Na⁺/K⁺-ATPase Ca²⁺-ATPase free amino acid

Received: 24 December 2019

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	Articles by authors
	XIONG Dalin
	DUAN Yafei
	CHEN Chengxun
	SUN Yongxu
	DONG Hongbiao
	ZHAN Aijun
	ZHANG Jiasong

Cite this article:

XIONG Dalin,DUAN Yafei,CHEN Chengxun, et al. Combined Effects of High Temperature and Ammonia Stress on Osmoregulation of Pacific White Shrimp Litopenaeus vannamei[J]. Fisheries Science, 2021, 40(4): 475-482.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.19281 OR https://www.shchkx.com/EN/Y2021/V40/I4/475

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