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| Antimicrobial Susceptibility in Escherichia coli Isolated from Fishery Products in Zhanjiang |
| DING Xiuqiong1, HUANG Wu1, LIU Jianfang1, LEI Xiaoling2,3,4, MAI Jiahui2, HUANG Jianyong2, NIE Fanghong2,3,4 |
1. Technology Center, Zhanjiang Custom, Zhanjiang 524000, China;
2. College of Food Science and Technology,Guangdong Ocean University, Zhanjiang 524088, China;
3. Guangdong Provincial Key Laboratory of AquaticProduct Processing and Safety, Guangdong Ocean University, Zhanjiang 524088, China;
4. Key Laboratory of Advanced Processing of Aquatic Product of Guangdong HigherEducation Institution, Zhanjiang 524088, China |
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Abstract Antimicrobial susceptibility of 52 susceptible Escherichia coli strains isolated from fresh samples such as oyster Crassostrea ariakensis, green mussel Perna viridis, Manila clam Ruditapes philippinarum, hard clam Meretrix meretrix, and scallop Mimachlamys nobilis, and frozen samples of fish fillets and cooked shrimp to 19 antibacterial drugs including penicillins, quinolones, monocyclic lactams and sulfonamides by using K-B paper diffusion method, and then extended-spectrum β-lactamase (ESBLs) and plasmid-mediated quinolone resistance (PMQR) genes were amplified by PCR to detect the resistance of E. coli in fishery products in Zhanjiang. Results showed that all of the 52 E. coli strains were resistant to more than one antibiotic, with higher resistance rate of erythromycin(90.4%), sulfamethoxazolethe(55.8%), trimethoprim (53.8%), and cefotaxime (51.9%) than that in other antibiotics. About 76.9% of E. coli showed multiple resistant of up to 9 categories of antibiotics, and 10 strains were confirmed to be ESBLs bacteria,with detection rate of 19.2% (10/52), 8 strains carrying gene blaTEM, 7 strains carrying gene blaSHV, without blaCTX and blaOXA genes. It was confirmed that 15 strains were PMQR bacteria, with detection rate of 28.8% (15/52), the all carrying qnrA, aac (6′)-Ib-c and qepA genes, 9 strains carrying qnrS gene, 8 strains carrying qnrD gene, and only 1 strain carrying oqxB gene, without qnrB, qnrC and oqxA genes. Both ESBLs gene and PMQR gene were carried by 2 E. coli isolates. The findings revealed that E. coli from fishery products in Zhanjian area was resistant to a wide range of antibiotics and that combination of ESBLs gene with PMQR gene enhanced the drug resistance of bacteria.
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Received: 16 January 2020
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[1]王高朋.青霉素在近代中国的传播与接受[D].保定:河北大学,2018.
[2]O′Neill J. Tackling drug-resistant infections globally: final report and recommendations[R].London:Government of the United Kingdom,2016.
[3]Marshall B M, Levy S B. Food animals and antimicrobials:impacts on human health[J].Clinical Microbiology Reviews,2011,24(4):718-733.
[4]沈应博,史晓敏,沈建忠,等.全基因组测序与生物信息学分析在细菌耐药性研究中的应用[J].生物工程学报,2019,35(4):541-557.
[5]苑丽.鸡源分离菌超广谱β-内酰胺酶基因分型和整合子分子特征[D].郑州:河南农业大学,2010.
[6]刘五高,丁友法,刘爱霞,等.质粒介导的耐药基因水平传播研究进展[J].中国卫生检验杂志,2015,25(8):1288-1292.
[7]Martínez-Martínez L, Pascual A, Jacoby G A. Quinolone resistance from a transferable plasmid [J]. Lancet,1998,351(9105):797-799.
[8]Nishikawa R, Murase T, Ozaki H. Plasmid-mediated quinolone resistance in Escherichia coli isolates from commercial broiler chickens and selection of fluoroquinolone-resistant mutants[J].Poultry Science,2019,98(11):5900-5907.
[9]Li P, Liu D, Zhang X Z, et al. Characterization of plasmid-mediated quinolone resistance in gram-negative bacterial strains from animals and humans in China[J].Microbial Drug Resistance,2019,25(7):1050-1056.
[10]Kilani H, Ferjani S, Mansouri R, et al. Occurrence of plasmid-mediated quinolone resistance determinants among Escherichia coli strains isolated from animals in Tunisia:specific pathovars acquired qnr genes[J].Journal of Global Antimicrobial Resistance,2020,20:50-55.
[11]章卓亮,金秀萍,茅国峰,等.大肠埃希菌耐药特征及质粒介导喹诺酮耐药基因分布[J].中国卫生检验杂志,2019,29(10):1172-1174.
[12]El-Badawy M F, Alrobaian M M, Shohayeb M M, et al. Investigation of six plasmid-mediated quinolone resistance genes among clinical isolates of Pseudomonas:a genotypic study in Saudi Arabia[J].Infection and Drug Resistance,2019,12:915-923.
[13]姜晓冰.大肠杆菌质粒介导喹诺酮耐药机制研究[D].广州:华南理工大学,2013.
[14]Seo K W, Lee Y J. Detection of plasmid-mediated quinolone resistance genes in β-lactamase-producing Escherichia coli isolates from layer hens[J].Poultry Science,2019,98(3):1480-1487.
[15]王彬婷,夏菁,黄运芳,等.合肥地区动物源致病性大肠杆菌ESBLs和PMQR基因流行分布[J].中国兽医学报,2013,33(4):581-585.
[16]Hui Y H, Kitts D, Stanfield P S. Foodborne disease handbook, volume Ⅳ: seafood and environmental toxins[M]. 2nd ed. New York:CRC Press,2018:121-126.
[17]教郁,高维凡,胡彩光.大肠杆菌耐药性研究进展[J].现代畜牧兽医,2013(5):53-58.
[18]杨微.大肠杆菌耐药性的研究进展[J].畜牧与饲料科学,2011,32(6):116-118.
[19]Schwarz S, Chaslus-Dancla E. Use of antimicrobials in veterinary medicine and mechanisms of resistance[J].Veterinary Research,2001,32(3/4):201-225.
[20]Adelowo O O, Fagade O E, Agersø Y. Antibiotic resistance and resistance genes in Escherichia coli from poultry farms,southwest Nigeria[J].Journal of Infection in Developing Countries,2014,8(9):1103-1112.
[21]Word Health Organization. WHO global strategy for containment of antimicrobial resistance [R].Geneva:World Health Organization,2001.
[22]刘永涛,何雅静,房金岑,等.动物源性水产品中兽药残留限量标准现状及对比分析[J].中国渔业质量与标准,2019,9(6):1-17.
[23]李爱华.水产养殖中使用的抗菌药物及细菌耐药性[J].中国水产科学,2002,9(1):87-91.
[24]王瑞旋,冯娟,耿玉静,等.水产细菌耐药性的最新研究概况[J]. 海洋环境科学,2010,29(5):770-776.
[25]杨虹,苗鹏飞,谭淑雯,等.类志贺邻单胞菌耐药基因检测与耐药性关系的研究[J].水产科学,2019,38(2):266-270.
[26]李琴,孙成波,陈美领,等.湛江东海岛对虾育苗场水体细菌的抗生素耐药性[J].热带生物学报,2014,5(1):8-14.
[27]汤电,张小华,付晓平,等.广东地区鱼源大肠埃希菌ESBLs和PMQR流行分布调查[J].华南农业大学学报,2012,33(1):113-119.
[28]余丽,熊丽娜,石磊,等.广州市售鱼、虾、蛤蚌中四环素耐药菌及四环素耐药基因的研究[J].现代食品科技,2016,32(10):239-245.
[29]叶蕾.广州市水产养殖品中耐药共生菌分布及耐药基因传播机制的研究[D].广州:华南理工大学,2012.
[30]张梦寒,沈强,朱莉勤,等.水产品中大肠埃希菌耐药性研究[J].中国卫生检验杂志,2011,21(10):2543-2545,2548.
[31]中华人民共和国卫生部.GB 4789.38—2012,食品安全国家标准 食品卫生微生物学检验 大肠埃希氏菌计数[S].北京:中国标准出版社,2012.
[32]Clinical Laboratory Standards Institute. M100-S23, Performance standards for antimicrobial susceptibility testing, twenty-third informational supplement [S]. Wayne, PA:Clinical Laboratory Standards Institute,2013.
[33]Guo X, Xia R, Han N, et al. Genetic diversity analyses of class 1 integrons and their associated antimicrobial resistance genes in Enterobacteriaceae strains recovered from aquatic habitats in China[J].Letters in Applied Microbiology,2011,52(6):667-675.
[34]Briñas L, Zarazaga M, Sáenz Y, et al. Beta-lactamases in ampicillin-resistant Escherichia coli isolates from foods, humans, and healthy animals[J].Antimicrobial Agents and Chemotherapy,2002,46(10):3156-3163.
[35]Robicsek A, Strahilevitz J, Sahm D F, et al. Qnr prevalence in ceftazidime-resistant Enterobacteriaceae isolates from the United States[J].Antimicrobial Agents and Chemotherapy,2006,50(8):2872-2874.
[36]Wang M H, Guo Q L, Xu X G, et al. New plasmid-mediated quinolone resistance gene,qnrC,found in a clinical isolate of Proteus mirabilis[J].Antimicrobial Agents and Chemotherapy,2009,53(5):1892-1897.
[37]Cavaco L M, Hasman H, Xia S, et al. qnrD, a novel gene conferring transferable quinolone resistance in Salmonella enterica serovar Kentucky and Bovismorbificans strains of human origin[J].Antimicrobial Agents and Chemotherapy,2009,53(2):603-608.
[38]Park C H, Robicsek A, Jacoby G A, et al. Prevalence in the United States of aac(6′)-Ib-cr encoding a ciprofloxacin-modifying enzyme[J].Antimicrobial Agents and Chemotherapy,2006,50(11):3953-3955.
[39]Cattoir V, Poirel L, Nordmann P. Plasmid-mediated quinolone resistance pump QepA2 in an Escherichia coli isolate from France[J].Antimicrobial Agents and Chemotherapy,2008,52(10):3801-3804.
[40]Chen X, Zhang W Q, Pan W J, et al. Prevalence of qnr, aac(6′)-Ib-cr, qepA, and oqxAB in Escherichia coli isolates from humans, animals, and the environment[J].Antimicrobial Agents and Chemotherapy,2012,56(6):3423-3427.
[41]贾敏.克氏原螯虾产ESBLs大肠杆菌耐药性研究[D].长春:吉林农业大学,2016.
[42]Martínez-Martínez L, Pascual A, García I, et al. Interaction of plasmid and host quinolone resistance[J].The Journal of Antimicrobial Chemotherapy,2003,51(4):1037-1039.
[43]Wang M G, Tran J H, Jacoby G A, et al. Plasmid-mediated quinolone resistance in clinical isolates of Escherichia coli from Shanghai, China[J].Antimicrobial Agents and Chemotherapy,2003,47(7):2242-2248.
[44]陈聪.40株PMQR基因阳性大肠埃希菌和肺炎克雷伯菌中ESBLs流行特征的研究[D].合肥:安徽医科大学,2013. |
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