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| Characterization, Expression and Function of SARM in Sea Cucumber Apostichopus japonicus |
| SUN Hongjuan1, CHEN Zhong1, HE Di2, GAO Shan1, WANG Bai1, GUAN Xiaoyan1, JIANG Jingwei1, DONG Ying1, ZHOU Zunchun1 |
1.Liaoning Key Laboratory of Marine Fishery Molecular Biology, Liaoning Ocean and Fisheries Science Research Institute, Dalian 116023, China;
2.Linghai Dalian Marine Treasures Breeding Co., Ltd., Jingzhou 121209, China |
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Abstract SARM, an adaptor of MyD88 independent signal pathway, was cloned in sea cucumber Apostichopus japonicus, named AjSARM by RACE method. The full length of AjSARM was 3874 bp with an ORF of 2412 bp encoding 803 amino acids. Structure prediction by SMART indicated that AjSARM contained two sterile motifs (SAM) and a C-terminal TIR domain. Quantitative real-time PCR was selected to examine AjSARM expression at different developmental stages of sea cucumber. AjSARM was rapidly up-regulated from auricularia at nervous development stage. It was indicated that AjSARM played an important role in the process of development. After four pathogens stimulus, the expression of AjSARM was inhibited at 4 h and 24 h in the groups of Vibrio splendidus, Pseudoalteromonas nigrifacien and Shewanella baltica chanlenged, but upregulated at 24 h in Bacillus cereus group. Except in S. baltica group, AjSARM reached top expression level all in other three groups at 96 h. The dynamic expression patterns of AjSARM at different times in response to different pathogens proved that it participated in the immune reaction in sea cucumber coelomocytes. The structure and expression analyses of AjSARM help to explore its functions as an adaptor and will lay foundations for the evolution study of immune system in echinoderm and disease prevention in sea cucumber.
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Received: 10 February 2020
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