养殖可口革囊星虫纤溶酶分离纯化及酶学特性研究

doi:10.16378/j.cnki.1003-1111.20053

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Abstract The fibrinolytic enzyme was extracted from coelomic fluid, intestine, esophagus of cultured sipunculid Phascolosoma esculenta by precipitation, Q sepharose fast flow anion-exchange and sephcry1 S-100 gel filtration, and located in the intestine. Effects of pH (3, 4, 5, 6, 7, 8, 9, and 10), temperature (4 ℃, 37 ℃, 50 ℃, 60 ℃, and 70 ℃), metal ions (Na⁺, K, Ca²⁺, Mg²⁺, Fe²⁺, Cu²⁺, and Al³⁺) on enzyme activity and ability to dissolve blood clots in vitro were investigated in the fibrinolytic enzyme of sipunculid. The results showed that a fibrinolytic enzyme extracted from intestine of sipunculid known as PK had strong ability to dissolve fibrinolytic protein, with relative molecular mass of about 32 ku, and specific activity of 4343.12 U/mg, increased by 13.82 folds compared with that in crude extract enzyme. But only 3.83% of total enzyme activity recovery was observed. The stable activity of the PK, with blood clot solubility of 88.09%, was kept at pH from 6 to 9 and below 37 ℃, and activated by Mg²⁺ (P＜0.05) and inhibited by Fe²⁺, Cu²⁺, and Al³⁺ (P＜0.05). The findings indicated that there was fibrinolytic enzyme activity in the intestine of sipunculid, which provided reference with development of fibrinolytic enzyme.

Key words： sipunculid Phascolosoma esculenta fibrinolytic enzyme enzymatic property purification

Received: 27 March 2020

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	Articles by authors
	CAI Binxin
	ZHOU Fengfang
	RUAN Shaojiang
	HUANG Weiqing
	LIU Congying
	SU Yu

Cite this article:

CAI Binxin,ZHOU Fengfang,RUAN Shaojiang, et al. Isolation and Characterization of a Fibrinolytic Enzyme from Cultured Sipunculid Phasclosoma esculenta[J]. Fisheries Science, 2021, 40(6): 922-928.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.20053 OR https://www.shchkx.com/EN/Y2021/V40/I6/922

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