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| Effects of Different Intensive Grass Carp Culture Modes on Aeromonas Community |
| XU Xiandong1,2, FU Huiyun2, OUYANG Min2, WAN Guoyuan3, RAO Yi2, HUANG Jiangfeng2, ZHOU Zhiyong2, LIU Lihui1 |
1. Key Laboratory of Fishery Drug Development, Ministry of Agriculture and Rural Affairs, Key Laboratory of AquaticAnimal Immune Technology, Guangdong Province, Guangzhou 510380, China;
2. Jiangxi Fisheries ResearchInstitute, Nanchang 330039, China;
3. Apiculture Institute of Jiangxi Province, Nanchang 330201, China |
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Abstract The abundance and diversity of Aeromonas bacteria were investigated in water samples collected at 15 cm of the surface at both ends and the middle of the pond, and sediment samples in three culture modes of grass carp Ctenopharyngodon idellus ponds including intensive formulated feed pond, ryegrass and other forages mountain pond, and grass carp, fish and vegetable symbiosis (aquaponic) pond from May to July 2017 (the period of vigorous breeding and feeding) by RS plate separation method and by 16S rDNA sequencing. The results showed that a total of 54 strains of bacteria were isolated, and divided into 10 genera and 22 species, with 23 Aeromonas strains, 8 Pseudomonas strains, 6 Citrobacter strains, 7 Enterobacter strains, 3 Pantoea strains and 3 Serratia strains. There was 1 strain of bacteria in genera such as Pectinobacterium, Salmonella, Kluyverella and Leclercia, with the maximal number of Aeromonas veronii (12 strains), followed by A. hydrophila (10 strains). There were 6 strains of E. tabaci, 5 strains of C. freundii, and 1 or 2 of other strains. The analysis of bacterial species and quantity in different modes revealed that there was the most bacterial species and quantity (12 species and 21 strains), and the least pathogenic bacteria in the aquaponics system. The least bacterial species (5 species and 13 strains) and higher proportion of pathogenic bacteria was observed in the intensive grass carp culture pond with commercial feed. The distribution characteristics of bacteria showed that A. veronii was mostly distributed in water, and A. hydrophila was mostly found in sediment.
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Received: 31 March 2020
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