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| Genetic Diversity among Six Geographical Populations of Schlegel's Black Rockfish Sebastes schlegelii by Microsatellite Marker |
| REN Jiangong1, WANG Qinglin2, SUN Zhaohui1, YU Shanshan3, WANG Guixing1, YU Qinghai1, LIU Xia1, SONG Limin4, SI Fei1 |
1. Beidaihe Central Experiment Station, Chinese Academy of Fishery Sciences, Qinhuangdao 066100, China;
2. College of Animal Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao 066600, China;
3. Ocean College, Agriculture University of Hebei, Qinhuangdao 066003, China;
4. Tianjin Fisheries Research Institute, Tianjin 300221, China |
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Abstract Genetic diversity, genetic structure, and genetic composition were investigated in 180 samples of six geographical populations of Schlegel's black rockfish Sebastes schlegelii collected from Dandong and Yingkou in Liaoning province, Beidaihe in Hebei province and Changdao, Weihai, and Rizhao in Shandong province in order to understand the impact of large-scale release on the genetic diversity of Schlegel's black rockfish in the Yellow Sea and Bohai Sea by 10 pairs of microsatellite markers. The results showed that there was mean allele from 17.200 to 19.900 in each locus, with average observed heterozygosity ranging from 0.769 to 0.923, the average expected heterozygosity ranging from 0.871 to 0.920, and the average polymorphic information content varying from 0.845 to 0.897. Hardy-Weinberg Equilibrium indicated that 15 loci were proved significantly deviated from 5 groups (P<0.05) with Gst values ranging from 0.019 to 0.093, and the average value of 0.039 among the six populations. AMOVA showed that most of the genetic variance was explained by the differences within individuals (93.078%), a few of differences derived from individuals within populations (3.975%). Nei's genetic distance and cluster analysis indicated that there was the maximal genetic distance between the Beidaihe population and the other five groups, being separated from each other. Both Dandong population and the Weihai population were clustered together first, and then they got into one with the Changdao population, and the Rizhao population and the Yingkou population were both gathered together. Three genetic compositions were observed in the six populations. The genetic information in the Dandong population and the Weihai population was intact and was not mixed. While the Yingkou population and the Rizhao population was significantly mixed genetically. The findings indicated that the six geographical populations of Schlegel's black rockfish had high genetic diversity, without obvious genetic diversity effect of releasing large number of hatchery-reared fish on wild Schlegel's black rockfish.
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Received: 31 March 2020
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