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| Effects of Different Lipid Levels Diet Supplemented with Surfactin in Orange Spotted Grouper Epinephelus coioides |
| ZHU Huaining1, SUN Xiuwen1, ZHAI Shaowei1,2 |
1. Fishery College of Jimei University, Xiamen 361021, China;
2. Eel Engineering Research Center of the Modern Industry Technology, Ministry of Education, Xiamen 361021, China |
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Abstract The 56 days feeding trail was conducted to investigate the effects of different lipid levels diet supplemented with surfactin in orange spotted grouper Epinephelus coioides. A total of 480 healthy juvenile grouper with the mean body weight of (23.36±0.06) g were randomly assigned to six treatments with four replicates per treatment and 20 fish per replicate. Six experimental diets were formulated to contain three lipid levels (6%,10% and 14%) and two surfactin supplemental levels (0 mg/kg and 100 mg/kg). Compared with 6% lipid group and 10% lipid group without surfactin supplementation, the weight gain rate and specific growth rate of those two groups with surfactin supplementation were significantly improved (P<0.05), and similar with the parameters in the 14% lipid group without surfactin supplementation (P>0.05); the feed conversion ratio and food intake were significantly decreased (P<0.05). When the dietary lipid level was 14%, the above growth performance parameters were not significantly affected by surfactin supplementation (P>0.05). The intestinal lipase activity were significantly increased by surfactin supplementation at different lipid levels (P<0.05), the activities of amylase and protease were not significantly affected (P>0.05). The content of total cholesterol were decreased significantly (P<0.05), the content of high-density lipoprotein cholesterol increased significantly (P<0.05), and the level of low-density lipoprotein cholesterol were not significantly affected by the surfactin supplementation (P>0.05), while the level of triglyceride was decreased significantly by surfactin supplementation at 10% and 14% lipid levels (P<0.05). In conclusion, dietary surfactin supplementation improved the activity of lipase in intestine, blood lipid level and growth of juvenile grouper at 6% and 10% lipid levels, and the growth performance were not significantly affected at 14% lipid level.
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Received: 30 April 2020
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