急性高温胁迫对大口黑鲈“优鲈3号”组织损伤及HSPs基因表达的影响

doi:10.16378/j.cnki.1003-1111.20095

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Abstract Juvenile largemouth bass Micropterus salmoides “Youlu No.3” with body weight of (9.87±1.2) g held at 25 ℃ (control group) was directly exposed to the water environment at 28, 31, 34, and 37 ℃ and sampled at 0 h, 6 h, and 48 h to investigate the effects of temperature on structural damage of liver and gill tissues and expression of HSPs gene in liver and gill of largemouth bass “Youlu No.3”. The results showed that no differences in structure of the liver and gill tissues were observed in the 28 ℃ and 31 ℃ groups between the treated and control largemouth bass “Youlu No.3”. However, there was slight cell edema in liver and gill tissues at 34 ℃, and the liver cells showed increase in vesicles structure, the messy and blurred, and the nucleus dissolved boundaries between cells at 37 ℃. Meanwhile, the gill lamellae became overall edema and severely bending, some swollen gill lamellae due to too many red blood cells. The expression levels of HSPs gene were found to be varied with changes in different water temperatures. At 6 h, the mRNA expression levels of HSP70 and HSC70 in liver and gill tissues were increased with the increase in stress temperature, with the maximal expression levels of HSP70 and HSC70 at 37 ℃. At 48 h, the mRNA expression levels of HSP70 and HSC70 in liver and gill tissues were decreased to some extent compared to that at 6 h (except for HSP70 at 31 ℃ gill tissue). The mRNA expression levels of HSP70 and HSC70 in liver and gill tissues showed a trend of first increasing and then decreasing, with the minimal level in the 37 ℃ group. The findings indicated that high temperature stress led to some stress damage to largemouth bass “Youlu No.3”. In aquaculture practices, attention should be paid to changes in temperature to reduce the impact of temperature stress on the immune function of fish.

Key words： Micropterus salmoides “Youlu No.3” acute high temperature stress tissue damage HSP70 HSC70

Received: 30 April 2020

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	LU Jian
	ZHANG Jiajia
	ZHOU Guoqin
	WANG Peipei
	QING Hui

Cite this article:

LU Jian,ZHANG Jiajia,ZHOU Guoqin, et al. Effect of Acute High Temperature Stress on Tissue Damage and HSPs Gene Expression of Largemouth Bass Micropterus salmoides “Youlu No.3”[J]. Fisheries Science, 2021, 40(4): 508-515.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.20095 OR https://www.shchkx.com/EN/Y2021/V40/I4/508

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