低盐胁迫下金乌贼转录组学分析

doi:10.16378/j.cnki.1003-1111.2020.03.009

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Abstract The transcriptome data were determined in juvenile cuttlefish Sepia esculenta with body weight of (1.3±0.3) g exposed to normal salinity (30) and low salinity stress (15) for 6 h by high throughput sequencing to investigate the molecular mechanism of low salinity stress on the cuttlefish. A total of 87 326 026 sequences were obtained by sequencing, and 575 171 transcripts and 513 053 Unigenes were obtained through the quality shear and reassembly of the raw data. A total of 62 485 annotation results were obtained in the NR, Swiss-Prot, KEGG, String, and Pfam databases. KEGG pathways with a larger number of Unigenes were purine, pyrimidine and carbon metabolism, PI3K-AKT, cAMP and Rap1 signaling pathways, endocytosis, RNA transport, focal adhesion, lysine degradation, and ubiquitin mediated protein hydrolysis. Low salinity stress led to produce 1923 differential expression genes. GO functional enrichment analysis revealed that some biological processes related to low salinity stress, such as α-amino acid, carboxylic acid, oxyacetic acid, organic acid and RNA metabolism, were significantly enriched. GO visualization analysis indicated that low salinity stress had a significant impact on the binding of metal ions, anions and nucleotides, α-amino acid metabolism, and hydrolase activity. The enrichment analysis of the KEGG pathway showed that the difference expression genes under low salinity stress of 6h mainly enriched into estrogen and cardiomyocyte epinephrine signaling pathways, steroid biosynthesis, antigen processing and expression, fat and protein digestion and absorption, glycerin lipids, arachidonic acid and tyrosine metabolism. The gene and pathway information found in this study provides technical support for exploring the physiological mechanism of low salinity stress of cuttlefish, for developing molecular markers and for cloning of key genes in the future.

Key words： Sepia esculenta low salinity stress transcriptome sequencing bioinformatics analysis differential gene screening

Received: 11 March 2019

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	Articles by authors
	WANG Xingqiang
	CAO Mei
	CUI Chunhui
	ZHENG Nianhao
	SHEN Ye
	LIU Haojie
	DING Xuefeng

Cite this article:

WANG Xingqiang,CAO Mei,CUI Chunhui, et al. Transcriptome Analysis of Cuttlefish Sepia esculenta Exposed to Low Salinity Stress[J]. Fisheries Science, 2020, 39(3): 369-380.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.2020.03.009 OR https://www.shchkx.com/EN/Y2020/V39/I3/369

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