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| Toxicity of Copper Sulfate and Zinc Chloride on DNA Damage and Metallothionein Gene Expression in Loach Cell Lines |
| JIANG Shan, LI Xia, LI Zhuangzhuang, YANG Yanjin, WANG Yu, ZHU Yuanchen, QIN Yanjie |
| Key Laboratory of Marine Bio-resource Restoration and Habitat Reparation in Liaoning Province, Key Laboratory of Mariculture and Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs, Dalian Ocean University, Dalian 116023, China |
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Abstract The effects of copper sulfate and zinc chloride on DNA damage and metallothionein (MT) gene expression were studied in loach fin cell lines in this study. Cells were suffered by 0, 100, 200, 400 and 800 μmol/L copper sulfate, and 0, 200, 400, 800 and 1600 μmol/L zinc chloride, respectively, for 24 h, and the DNA damage (detected by single cell gel electrophoresis)and MT expression were studied. Results showed that the DNA damage were gradually obvious with the increasing concentrations of copper sulfate and zinc chloride, showing concentration dependent characteristics. When copper sulfate and zinc chloride were greater than or equal to 200 μmol/L and 400 μmol/L, respectively, the Tailing percent, comet tailing DNA ratio (Tail DNA), comet tail length (L Tail), comet tail distance (TM) and Olive tail moment (OTM) were significantly increased(P<0.05). MT mRNA expression levels were studied by Real-time PCR using β-actin as internal reference gene. The MT mRNA expression values were maintained at the level of the control group, and then decreased significantly with the increasing concentration of copper sulfate. Expressed levels of MT in loach cells increased at low concentration of zinc chloride (200 and 400 μmol/L) and then decreased significantly with the increasing zinc chloride concentration. These findings indicated that MT mRNA were induced at zinc chloride concentration of less than or equal to 400 μmol/L. In general, loach fin cells were more sensitive to copper sulfate than to zinc chloride, and the genetic toxicity was more obviously in the cells exposed to copper. The results of this study will allow us to understand the mechanism of genetic toxicity to loach cells under Cu and Zn exposure and provide some information about physiology regulation and environmental monitoring.
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Received: 29 September 2019
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