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| Toxicological Effect of Ammonia on Embryos and Prolarvae of Taiwan Loach |
| WANG Mengjie1,2, WANG Haihua2, MA Benhe2, CHEN Jianhua1, ZHU Ming1 |
1. Jiangsu Key Laboratory of Marine Bioresources and Environment, College of Marine Life and Fisheries, Jiangsu Ocean University, Lianyungang 222005, China;
2. Scientific Observing and Experimental Station of Fishery Resources and Environment in Poyang Lake, Ministry of Agriculture and Rural Affairs, Fisheries Research Institute of Jiangxi Province, Nanchang 330039, China |
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Abstract The blastula embryos of Taiwan loach(Paramisgurnus dabryanus ssp. Taiwan) were placed in a 90 mm diameter, 45 mm high glass crystal dish and exposed to 0(control), 5, 10, 20, 40, and 80 mg/L ammonia concentrations in order to investigate the effects of different concentrations of ammonia on the development and toxicity of embryos and prolarvae of Taiwan loach. The hatching success, incubation period, mortality rate, abnormality rate, body length, heart beating rate, yolk absorption rate and specific growth rate were measured under a microscope. The embryo hatching rate, mortality rate and larva malformation rate of Taiwan loach were found to be significantly increased with the increase in ammonia concentration(P<0.05), without significant effects of ammonia exposure on the incubation period and embryonic heart beating rate(P>0.05). All of the dead prolarvae were observed in 40 and 80 mg/L concentration groups within 48 h and 12 h after hatching, respectively. The growth and yolk absorption rate of larval Taiwan loach exposed to high ammonia concentration were inhibited (P<0.05). However, there was no significant difference in embryonic development and larva growth between the low concentration group (5 and 10 mg/L) and the control group(P>0.05). The findings explored the toxicological effects of ammonia exposure on embryo-larvae of Taiwan loach, and provided theoretical basis for breeding of Taiwan loach.
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Received: 29 July 2019
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