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| Effects of Vitamin C on Resistance to Dimethoate Stress in Juvenile Schizothorax grahami |
| YANG Tongzhi1, ZHAN Huixiang2, YAO Junjie1, YANG Mei1, WEI Yulai1, YANG Longbao1 |
1. College of Animal Sciences, Guizhou University, Guiyang 550025, China;
2. Aquaculture Techniques Extension Station of Bijie, Guizhou Province, Bijie 551700, China |
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Abstract 40% Dimethoate was added to the mountain spring water in a tank with culture of Schizothorax grahami with body weight of (1.52±0.26) g at a dose of 0 (control group), 5.091, 10.182, 24.89, and 39.598 mg/L (96 h LC50=39.598 mg/L, safe concentration SC=10.182 mg/L) at water temperature of (13.5±0.5) ℃ for 120 h, and then 90 mg/L of vitamin C was added to water body in each group in order to investigate the toxicity of dimethoate to juvenile S. grahami, the toxic environment simulated by injecting dimethoate into the aquaculture water. The changes in activities of Fethoxy resorufin-deethylase (EROD), AchE, and catalase (CAT) and malondialdehyde (MDA) and the expression levels of HSP70 gene in juvenile S. grahami under dimethoate stress were investigated, and the detoxification effect of vitamin C on the stress was further explored. Activities of EROD, acetylcholinesterase and MDA content were analyzed in the muscle, liver, and gills of Schizothorax grahami in each group collected at 0, 24, 48, 96, and 120 h, and the expression of HSP70 mRNA and gene cloning in another muscle samples in a 5 to 10-fold volume of RNA preservation solution to investigate the ability of vitamin C to resist dimethoate stress in juvenile S. graham. The results showed that there were significantly increase in activities of EROD, AchE, and CAT and MDA level in juvenile S. graham in the over SC groups (P<0.05), with the lower activities of EROD, CAT, and AchE and MDA level in the SC group and the less than SC groups after adding vitamin C, the higher activity of EROD, and lower activities of CAT, and AchE and MDA level were observed in the juvenile S. graham exposed to the water containing vitamin C. The expression of HSP70 mRNA was shown to be significantly induced by different concentrations of dimethoate (P<0.05), without significant change in the other groups (P>0.05), except the maximal concentration group from 24 h to 96 h. After 96 h, however, the expression level of HSP 70 gene was decreased significantly in each group (P<0.05), close to the control group in the two lower concentration groups. The findings indicated that the damage to the body by dimethoate was not found to be recovery at dimethoate concentration of over SC, to a certain extent, without effect on the body at dimethoate concentration of less than SC. Vitamin C led to an active role in protecting the body from oxidative damage caused by exogenous substances to a certain extent, even though dimethoate induced the expression level of HSP 70 gene in juvenile S. grahami fish.
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Received: 24 May 2019
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