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| Isolation, Identification and Characteristics of Pathogen from Sea Cucumber Apostichopus japonicus with Skin Ulceration Syndrome |
| LI Xiaoyu1,2,3, FENG Lijuan1, XU Le1, WANG Lili1,2,3, ZHANG Meixia1, LI Shuying3,4, LI Jibin3,4, XU Yongping1,2,3 |
1. School of Bioengineering, Dalian University of Technology, Dalian 116024, China;
2. Center for Food Safety of Animal Origin, Ministry of Education, Dalian 116600, China;
3. Professional Technology Innovation Center for Phage Application of Liaoning Province, Dalian 116600, China;
4. Dalian SEM Bio-Engineering Technology Co. Ltd., Dalian 116600, China |
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Abstract In order to explore the death causes of sea cucumber Apostichopus japonicus with skin ulceration from a local farm in Dalian, a dominant bacterial strain AP-1 was isolated from the diseased sea cucumbers. The 16S rDNA sequence, morphological observation, physiological and biochemical characteristics results showed that it was Vibrio alginolyticus. The strain was artificially infected back to the healthy sea cucumber by the means of immersion infection, body wall wound immersion infection and intraperitoneal injection infection, respectively. The results showed that the immersion infection did not show pathological symptoms, whereas other two methods showed different degrees of dose-dependent lethality. The LC50 of the sea cucumber with the isolate was 5.23×106 cfu/mL in body wall wound immersion infection and 3.08×104 cfu/g in intraperitoneal injection infection. Virulence gene detection showed that the strain was positively amplified with the seven virulence genes including toxR, FlaA, Collagenase, fur, AspA, ompW and tlh. Drug resistance analysis showed that the strain was resistant to ampicillin, piperacillin, augmentin, cefazolin, cefotaxime and amikacin, and was intermediate to cefuroxime, cefoxitin, chloramphenicol, aztreonam and neomycin. However, it was sensitive to other 16 antibiotics. This finding will provide scientific basis and reference for sea cucumber industry in Dalian.
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Received: 21 January 2021
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