|
|
|
| Application of Enzyme-Digested Hydrolyzed Porcine Mucosa in Diet of Hybird Grouper Epinephelus fuscoguttatus ♀×E. lanceolatu ♂ |
| ZHI Xinyan1,2, YANG Xuanyi1,2, WANG Guanghui3, ZHAO Xumin3, CHI Shuyan1,2,4, TAN Beiping1,4 |
1. College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China;
2. Guangdong Laboratory of Southern Ocean Science and Engineering, Zhanjiang 524013, China;
3. Yichang Huatai Biotechnology Co., Ltd., Yichang 443500, China;
4. Guangdong Engineering Research Center for Aquatic Animal Precision Nutrition and Efficient Feed, Zhanjiang 524088, China |
|
|
|
|
Abstract The purpose of this experiment was to study the effects of enzyme-digested hydrolyzed porcine mucosa (EPM) replacing fish meal on the growth performance, apparent digestibility coefficient of nutrients and aquaculture water quality in ponds withhybird grouper Epinephelus fuscoguttatus ♀×E. lanceolatu ♂. A total of 450 hybird grouper [initial body weight (7.50±0.02) g] was divided into 5 groups, each with 3 replicates, and each replicate with 30 fish was fed iso-nitrogenous and iso-lipidic diets with EPM replacement rates of 0%(EPM 0), 9%(EPM 9), 18%(EPM 18), 27%(EPM 27), and 36%(EPM 36) fish meal, respectively for 8-week. The results showed that the weight gain rate and specific growth rate in the EPM 9 group were not significantly different from that in the EPM 0 group (P>0.05), but were significantly higher than those in the other groups (P<0.05). The apparent digestibility coefficients of dry matter, crude protein, calcium and phosphoruswere significantly higher in EPM 0 group than those in the other groups (P<0.05). There was no significant difference in ammonia nitrogen content in aquaculture water between EPM 0 and EPM 9 groups with a period of 1 to 21 h after feeding (P>0.05). Twenty-one hours after feeding, there was no significant difference in the content of nitrite nitrogen in aquaculture water between the groups (P>0.05). In summary, when the substitution of 9% fish meal with enzyme-digested hydrolyzed porcine mucosa will not result in negative impact on the growth performance of the hybird grouper and the ammonia nitrogen content in aquaculture water.
|
|
Received: 28 April 2021
|
|
|
|
|
|
[1]农业农村部新闻办公室.2019年全国渔业经济统计公报[J].农产品市场,2020(13):36-37.
[2]许凤明.鱼粉质量及其在饲料行业的应用浅析[J].山东畜牧兽医,2020,41(7):26.
[3]单秀娟,韩冬,张文兵,等.中国水产养殖缓解了对野生渔业资源需求的压力[J].中国水产,2015(6):5-6.
[4]周歧存,麦康森,刘永坚,等.动植物蛋白源替代鱼粉研究进展[J].水产学报,2005,29(3):404-410.
[5]徐玲,徐宁迎.2010年国产鱼粉营养成分的比较分析[J].中国畜牧杂志,2011,47(12):58-61.
[6]陆静,邵庆均.水产动物对饲料中磷的利用研究进展[J].中国饲料,2013(1):34-38.
[7]唐武斌.烁生肽替代鱼粉对草鱼和黄颡鱼生长及水体水质的影响[D].武汉:华中农业大学,2008:37.
[8]吴瑞.复合蛋白KT67替代鱼粉对花鲈生长、消化和肠道健康的影响[D].厦门:集美大学,2018:14.
[9]周凡,邵庆均.不同蛋白源替代鱼粉的研究进展[J].饲料研究,2007(6):9-11.
[10]孟素宣,史东杰,秦佳,等.蝇蛆粉替代鱼粉对锦鲤生长、体组成、体色和非特异性免疫的影响[J].湖北农业科学,2016,55(8):2066-2070.
[11]朱旺明,谭永刚,马卉佳,等.不同水平南极磷虾粉等量替代鱼粉对大口黑鲈生长性能及部分生理生化指标的影响[J].中国饲料,2019(15):85-92.
[12]朱滔,黄小燕.肠膜蛋白在动物生产中应用研究进展[J].饲料研究,2015(20):9-13.
[13]江栋材,葛龙,王卫卫,等.肠膜蛋白粉的研究进展及在猪生产上的应用[J].中国猪业,2017,12(1):65-69.
[14]何姝祯.罗非鱼下脚料酶解物替代进口鱼粉养殖奥尼罗非鱼幼鱼效果研究[D].南宁:广西大学,2013:20.
[15]ZAMBONINO INFANTE J L, CAHU C L, PERES A. Partial substitution of di- and tripeptides for native proteins in sea bass diet improves Dicentrarchus labrax larval development[J].The Journal of Nutrition,1997,127(4):608-614.
[16]LI S L, LI Z Q, SANG C Y, et al. Glucose transporters in pearl Gentian grouper (Epinephelus fuscoguttatus ♀ × E. lanceolatus ♂):molecular cloning, characterization, tissue distribution and their expressions in response to dietary carbohydrate level[J].Aquaculture Research,2018,49(1):253-264.
[17]LEE M H. Official methods of analysis of AOAC International (16th edn)[J].Trends in Food Science & Technology,1995,6(11):382.
[18]国家教育委员会.JY/T 015—1996,感耦等离子体原子发射光谱方法通则[S].北京:中国标准出版社,1997:4.
[19]中华人民共和国国家质量监督检验检疫总局,中国国家标准化管理委员会.GB 17378.4—2007,海洋监测规范[S].北京:中国标准出版社,1999:109.
[20]曹晓莉,胡毅,胡亚军,等.饲料级鸡肉粉对黄鳝生长、消化酶及血清生化指标的影响[J].水产科学,2021,40(2):196-202.
[21]魏佳丽,王际英,宋志东,等.酶解磷虾粉替代鱼粉对珍珠龙胆石斑鱼幼鱼生长性能、体组成及血清生化的影响[J].渔业科学进展,2016,37(1):100-110.
[22]BUI H T D, KHOSRAVI S, FOURNIER V, et al. Growth performance, feed utilization, innate immunity, digestibility and disease resistance of juvenile red seabream (Pagrus major) fed diets supplemented with protein hydrolysates[J].Aquaculture,2014,418/419:11-16.
[23]周露阳.酶解虾浆、酶解鱼浆(粉)、鱼溶浆、酶解鱼溶浆和酶解鱼粉对黄颡鱼(Pelteobagrus fulvidraco)生长的影响[D].苏州:苏州大学,2019.
[24]ZHANG Y C, LI D F, FAN S J, et al. Effects of casein and protein-free diets on endogenous amino acid losses in pigs[J].Asian-Australasian Journal of Animal Sciences,2002,15(11):1634-1638.
[25]PLAKAS S M, KATAYAMA T. Apparent digestibilities of amino acids from three regions of the gastrointestinal tract of carp (Cyprinus carpio) after ingestion of a protein and a corresponding free amino acid diet[J].Aquaculture,1981,24:309-314.
[26]HA N, JESUS G F A, GONÇALVES A F N, et al. Sardine (Sardinella spp.) protein hydrolysate as growth promoter in South American catfish (Rhamdia quelen) feeding:productive performance, digestive enzymes activity, morphometry and intestinal microbiology[J].Aquaculture,2019,500:99-106.
[27]杜雪地,薛文,华杰,等.酶解鸡血粉替代鱼粉对异育银鲫生长性能及鱼体组成的影响[J].科学养鱼,2018(1):28-29.
[28]姜柯君.小肽对星斑川鲽(Platichthys stellatus)幼鱼生长、肌肉品质和生理生化指标的影响[D].上海:上海海洋大学,2013:25-26.
[29]曹素会.大菱鲆饲料中酶解羽毛粉替代鱼粉蛋白的可行性研究[D].上海:上海海洋大学,2020:3.
[30]DAVIES S J, GOUVEIA A, LAPORTE J, et al. Nutrient digestibility profile of premium (category Ⅲ grade) animal protein by-products for temperate marine fish species (European sea bass, gilthead sea bream and turbot)[J].Aquaculture Research,2009,40(15):1759-1769.
[31]CHI S Y, WANG W J, TAN B P, et al. The apparent digestibility coefficients of 13 selected animal feedstuff for cobia, Rachycentron canadum[J].Journal of the World Aquaculture Society,2017,48(2):280-289.
[32]张松,解绶启,朱晓鸣,等.粪便收集时间和饲料中肉骨粉含量对异育银鲫(Carassius auratus gibelio)消化率的影响[J].水生生物学报,2008,32(1):79-90.
[33]ZHOU Q C, TAN B P, MAI K S, et al. Apparent digestibility of selected feed ingredients for juvenile cobia Rachycentron canadum[J].Aquaculture,2004,241(1/2/3/4):441-451.
[34]杨艳玲,陈冰,彭凯,等.发酵豆粕替代鱼粉及添加包膜氨基酸、植酸酶对花鲈幼鱼生长性能及养殖水质的影响[J].饲料工业,2019,40(10):47-53.
[35]李雪.微生态菌对养渔水体水质及微生物群落结构的影响[D].沈阳:辽宁大学,2019:12-13.
[36]李波,樊启学,张磊,等.不同溶氧水平下氨氮和亚硝酸盐对黄颡鱼的急性毒性研究[J].淡水渔业,2009,39(3):31-35.
[37]李明.浅谈养殖水体中的氨氮[J].科学养鱼,2020(8):91.
[38]宋娇,姜海波,姜志强,等.混合植物蛋白替代鱼粉对杂交鲟幼鱼生长、排氨率和转氨酶活性的影响[J].水产科学,2016,35(2):99-104.
[39]KROUS S R, BLAZER V S, MEADE T L. Effect of acclimation time on nitrite movement across the gill epithelia of rainbow trout:the role of “chloride cells”[J].The Progressive Fish-Culturist,1982,44(3):126-130.
[40]运珞珈,李谷,刘志伟,等.稚鳖养殖水体中异养细菌及自养细菌的初步研究[J].同济医科大学学报,2000,29(5):397-399. |
|
|
|
