蒙脱石对仿刺参幼参生长、主要消化酶和免疫酶活性的影响

doi:10.16378/j.cnki.1003-1111.21075

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Abstract The juvenile sea cucumber Apostichopus japonicus with initial body weight of (1.37±0.07) g were reared in a 100 L plastic steel water tank at stocking density of 45 individuals per tank and fed diets containing 0%, 0.20%, 0.40%, 0.60% and 0.80% of montmorillonite for 75 days. Then the activities of intestinal protease and amylase and the activities of superoxide oxidase, lysozyme and alkaline phosphatase in coelomic fluid of the sea cucumber were determined at water temperature of 15.8—21.0 ℃ to probe into the effects of montmorillonite on growth performance, digestive enzyme activities, immunity and water quality in the juvenile sea cucumber culture tank. The results showed that montmorillonite supplement significantly enhanced the specific growth rate (SGR) of the juveniles, with the maximal SGR in the 0.40% supplement group (P<0.05), without significant differences in the survival rate (SR) among the groups (P>0.05). The juveniles fed the diets supplemented with montmorillonite had significantly high apparent digestibility of dry matter (P<0.05). The concentrations of montmorillonite supplement ranging from 0.20% to 0.60% led to significantly increase the activities of protease and amylase (P<0.05), and the maximum values [(73.27±3.73) U/mg and (70.63±1.96) U/mg, respectively] were both found in the 0.40% group. There were different activities of superoxide dismutase (SOD), lysozyme (LSZ) and alkaline phosphatase (AKP) in body fluid of the juveniles in different groups, with the maximal effect of montmorillonite supplement on the LSZ activity. The maximum activity of LSZ was observed in the 0.60% group, 50% higher than that in the control group. Therefore, the findings showed that diet supplemented with 0.40% montmorillonite improved the digestibility, immunity and growth of the sea cucumber, which provides foundation of optimizing the cultivation technology of sea cucumber.

Key words： Apostichopus japonicus montmorillonite growth performance apparent digestibility digestive enzyme immunity

Received: 28 April 2021

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	LI Junhua
	LIU Jialiang
	LI Jing
	SUN Luping
	LIU Shilin
	WANG Yuben

Cite this article:

LI Junhua,LIU Jialiang,LI Jing, et al. Effects of Dietary Montmorillonite on Growth Performance, Digestive Enzyme Activities, and Immunity of Juvenile Sea Cucumber Apostichopus japonicus[J]. Fisheries Science, 2023, 42(1): 96-101.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.21075 OR https://www.shchkx.com/EN/Y2023/V42/I1/96

[1]马玉龙,郭彤.载铜蒙脱石及其杀灭大肠杆菌机制的研究[J].药学学报,2007,42(3):318-322.
[2]刘鹏威,郭彤,魏华.纳米载铜蒙脱石体外对三种水产病原菌及两种肠道有益菌杀菌作用[J].上海海洋大学学报,2009,18(5):520-526.
[3]叶瑛,周玉航,夏枚生,等.新型无机抗菌材料:载铜蒙脱石及其抗菌机理讨论[J].无机材料学报,2003,18(3):569-574.
[4]ZHAO H Y, MAO X B, YU B, et al. Excess of dietary montmorillonite impairs growth performance, liver function, and antioxidant capacity in starter pigs[J].Journal of Animal Science,2017,95(7):2943-2951.
[5]XIA M S, HU C H, XU Z R, et al. Effects of copper-bearing montmorillonite (Cu-MMT) on Escherichia coli and diarrhea on weanling pigs[J].Asian-Australasian Journal of Animal Sciences,2004,17(12):1712-1716.
[6]徐子伟,万晶.饲料霉菌毒素吸附剂研究进展[J].动物营养学报,2019,31(12):5391-5398.
[7]HUWIG A, FREIMUND S, KÄPPELI O, et al. Mycotoxin detoxication of animal feed by different adsorbents[J].Toxicology Letters,2001,122(2):179-188.
[8]李旭,陈阳,杜银峰,等.蒙脱石在畜牧生产中的应用研究进展[J].饲料博览,2011(8):52-54.
[9]唐家.改性蒙脱石对断奶仔猪生长及抗腹泻效果的影响[D].长沙:湖南农业大学,2010.
[10]詹小立,应加富,邓波,等.改性蒙脱石对断奶仔猪生长性能、血清免疫和抗氧化功能及猪肠上皮细胞的影响[J].动物营养学报,2021,33(4):1944-1953.
[11]郭彤,马玉龙,许梓荣.纳米载铜蒙脱石对断奶仔猪生长、肠道菌群及其肠黏膜形态的影响[J].畜牧与兽医,2006,38(1):4-8.
[12]王芳,张海军,王晶,等.蒙脱石对采食霉变花生粕型饲粮肉仔鸡生长性能和免疫机能的影响[J].动物营养学报,2016,28(5):1480-1488.
[13]胡彩虹,徐勇,熊莉,等.载铜蒙脱石对尼罗罗非鱼肠道菌群和消化机能的影响[J].水生生物学报,2008,32(6):881-888.
[14]刘国栋,薛敏,李军国,等.杂交鲟对饲料中霉菌毒素吸附剂蒙脱石的耐受性评价[J].动物营养学报,2013,25(8):1886-1895.
[15]胡彩虹,夏枚生.载铜蒙脱石对水产病原菌的抗菌活性及其机理[J].硅酸盐学报,2005,33(11):1376-1380.
[16]HU C H, XU Y, XIA M S, et al. Effects of Cu²⁺-exchanged montmorillonite on growth performance, microbial ecology and intestinal morphology of Nile tilapia (Oreochromis niloticus)[J].Aquaculture,2007,270(1/2/3/4):200-206.
[17]刘志逊,苏茂凯.矿产资源在水产养殖中的应用[J].台湾海峡,1998,17(增刊):188-192.
[18]杨宁,郭中帅,王正丽.饲料中添加浒苔对仿刺参幼参生长、消化酶活性和免疫力的影响[J].水产科学,2016,35(5):498-503.
[19]王旭达,关晓燕,王鉴,等.地衣芽孢杆菌对仿刺参生长、消化和免疫功能的影响[J].水产科学,2021,40(2):151-158.
[20]王吉桥,唐黎,许重,等.仿刺参消化道的组织学及其4种消化酶活力的周年变化[J].水产科学,2007,26(9):481-484.
[21]ECKHARDT J C, SANTURIO J M, ZANETTE R A, et al. Efficacy of a Brazilian calcium montmorillonite against toxic effects of dietary aflatoxins on broilers reared to market weight[J].British Poultry Science,2014,55(2):215-220.
[22]唐小懿,马杰,张富群,等.蒙脱石和酵母培养复合物对断奶仔猪生长性能、血清生化指标、免疫功能及抗氧化能力的影响[J].动物营养学报,2018,30(9):3670-3680.
[23]SEO J Y, LEE S M. Optimum dietary protein and lipid levels for growth of juvenile sea cucumber Apostichopus japonicus[J].Aquaculture Nutrition,2011,17(2):e56-e61.
[24]XIA S D, YANG H S, LI Y, et al. Effects of different seaweed diets on growth, digestibility, and ammonia-nitrogen production of the sea cucumber Apostichopus japonicus (Selenka)[J].Aquaculture,2012,338/339/340/341:304-308.
[25]丁述理,彭苏萍,刘钦甫,等.膨润土用作饲料添加剂可行性分析[J].辽宁工程技术大学学报,2003,22(1):23-26.
[26]王黎文,丁健,张建刚,等.霉菌毒素吸附剂蒙脱石对泌乳奶牛生产性能和血清生化指标的影响[J].动物营养学报,2013,25(7):1595-1602.
[27]夏明亮,刘婕,齐德生,等.改性蒙脱石对肉鸡生产性能的影响[J].饲料工业,2012,33(10):55-59.
[28]张洪彩,朱江艳,陈政强,等.不同规格刺参消化酶活性差异性研究[J].泉州师范学院学报,2014,32(2):27-30.
[29]周兴华,向枭,王友慧.麦饭石在水产养殖中的应用[J].河南水产,2002(2):10-11.
[30]王聪,周凌云,董淑慧.蒙脱石主要作用机理及其在反刍动物生产中的应用研究进展[J].中国畜牧兽医,2012,39(8):108-111.