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| Effect of a High Protein Content Compound Feed Substitution for Trash fish on Intestinal Microbiota in Mud Crab Scylla paramamosain |
| SU Guisen1,2, LI Min1, CHEN Ran1, HU Gaoyu1, XIAO Guoqiang1,2, CAI Jingbo1, ZHANG Xiang1 |
1. Zhejiang Key Lab of Exploitation and Preservation of Coastal Bio-Resource, Wenzhou Key Laboratory of Marine Genetics and Breeding, Zhejiang Mariculture Research Institute,Wenzhou 325005,China;
2. National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China |
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Abstract To explore the differences of intestinal microbiota for Scylla paramamosain fed different diets (Eel feed-EF, and fresh fish-FF) under crab apartment, the microbial community structure and changes were studied by using the 16S rRNA gene amplicon sequencing technology in this study. The results showed that Proteobacteria, Firmicutes, Fusobacteria, and Bacteroidetes were the four most dominant categories at phylum level whose number of OTU accounted for more than 85.84%. The OTUs of common bacterial community accounted for 18.92% represented 95.39% of the sequences. The microbial community of different diets was no remarkably distinct by PCoA. With the pass of periods, the complexity of bacterial co-occurrence relationship decreased. The function analyses of intestinal microbiota showed that there was significant difference in secondary metabolites biosynthesis, transport and catabolism in early period, which was not significant in late period. Overall EF has no significant effect on the bacterial community structure and function.
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Received: 14 May 2021
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