3种环境因子对刚毛藻吸收氮的影响研究

doi:10.16378/j.cnki.1003-1111.21086

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Abstract Nitrogen is one of the important nutrients of aquatic plants. The utilization capacity of nitrogen plays an important role in the niche competition of aquatic plants. However, the utilization of nitrogen is also affected by environmental factors. In this study, we focused on Cladophora, which has important influence on culture, aiming to explore the influence of environmental factors on Cladophora nitrogen absorption, and provide ideas for nitrogen removal or biomass control. In this experiment, NH₄Cl was used as nitrogen source, and light incubator was used as culture condition. The nitrogen uptake of Cladophora was detected continuously. The time course and appropriate concentration for the stable absorption of NH₄⁺-N in Cladophora were determined. Accordingly, orthogonal experiment of temperature, pH and light intensity L9(3³) with different gradients was carried out. The results showed that Cladophora had a linear absorption of NH₄⁺-N (R²>0.99) when the concentration of NH₄⁺-N was 80 μmol/L within 0.5—1.5 h. The orthogonal experiment results showed that there wasa significant effect of temperature on the absorption of NH₄⁺-N in Cladophora (P<0.05), whereas no significant effects for light intensity and pH (P>0.05). The effect of the above three factors on the absorption rate of NH₄⁺-N in Cladophora was in such an order: temperature>pH> light intensity. The three-factor combination that was most conducive to the absorption in NH₄⁺-N of Cladophora was temperature of 25 ℃, pH of 9, and light intensity of 4500 lx. The correlation analysis of various enzymes, total antioxidant capacity and ammonia nitrogen absorption rate of Cladophora showed that environmental factors influenced nitrogen absorption by various enzymes and non-enzymes. The finding provides a scientific basis for scientific research, the purification of sewage by Cladophora and the prediction and inhibition of the occurrence of filamentous algae such as Cladophora.

Key words： Cladophora temperature light intensity pH ammonia nitrogen enzyme activity

Received: 14 May 2021

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	WANG Luping
	TANG Yongtao
	WANG Chen
	TANG Boping
	CHENG Yongxu

Cite this article:

WANG Luping,TANG Yongtao,WANG Chen, et al. Effects of Three Environmental Factors on Nitrogen Absorption of Filamentous alga Cladophora[J]. Fisheries Science, 2023, 42(5): 884-890.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.21086 OR https://www.shchkx.com/EN/Y2023/V42/I5/884

[1] LI Y G,LI Z K,GENG Y H,et al.Effect of N,P concentration on growth rate[J].Acta Ecologica Sinica,2006,26(2):317-325.
[2] CAO X Y,SONG C L,LI Q M,et al.Dredging effects on P status and phytoplankton density and composition during winter and spring in Lake Taihu,China[J].Hydrobiologia,2007,581(1):287-295.
[3] CHOO K S,SNOEIJS P,PEDERSÉN M.Oxidative stress tolerance in the filamentous green algae Cladophora glomerata and Enteromorpha ahlneriana[J].Journal of Experimental Marine Biology and Ecology,2004,298(1):111-123.
[4] ZULKIFLY S B,GRAHAM J M,YOUNG E B,et al.The genus Cladophora Kützing (Ulvophyceae) as a globally distributed ecological engineer[J].Journal of Phycology,2013,49(1):1-17.
[5] 张新龙,孙力平,钟远.刚毛藻对不同形态磷的去除[J].环境工程学报,2017,11(5):2743-2747.
[6] 杜娟娟,李粉婵.刚毛藻氮磷去除效果与培养方式试验研究[J].水资源开发与管理,2019,17(12):35-38.
[7] 陈孝花,潘连德,张饮江.水中丝状藻类有害藻华的形成与对策[J].南方水产科学,2011,7(2):77-82.
[8] 何义进.青苔的发生与防治[J].科学养鱼,2005(2):84.
[9] 李敦海,史龙新,李根保,等.丝状绿藻腐烂过程对水质和沉水植物黑藻生长的影响实验研究[J].环境科学与管理,2006,31(6):27-30.
[10] 张猛,石瑛,丁义晶.不同氮磷比和Fe³⁺浓度对刚毛藻生长繁殖的影响[J].江苏农业科学,2013,41(6):196-198.
[11] ROSS M E,DAVIS K,MCCOLL R,et al.Nitrogen uptake by the macro-algae Cladophora coelothrix and Cladophora parriaudii:influence on growth,nitrogen preference and biochemical composition[J].Algal Research,2018,30:1-10.
[12] 马沛明.利用着生藻类去除N、P营养物质的研究[D].武汉:中国科学院研究生院(水生生物研究所),2005.
[13] LELIAERT F,DE CLERCK O,VERBRUGGEN H,et al.Molecular phylogeny of the Siphonocladales (Chlorophyta:Cladophorophyceae)[J].Molecular Phylogenetics and Evolution,2007,44(3):1237-1256.
[14] 环境保护部.HJ 535—2009,水质氨氮的测定纳氏试剂分光光度法[S].北京:中国环境科学出版社,2010.
[15] 李俭平.浒苔对氮营养盐的响应及其氮营养盐吸收动力学和生理生态研究[D].青岛:中国科学院研究生院(海洋研究所),2011.
[16] 曹学彬,马伟伟,李琦,等.刚毛藻对静态模拟刺参养殖池塘上覆水营养盐含量的影响[J].海洋科学,2018,42(6):88-95.
[17] 方焰星,何池全,梁霞,等.水生植物对污染水体氮磷的净化效果研究[J].水生态学杂志,2010,31(6):36-40.
[18] 陈巧玲,林晓葱,宫本涛,等.12种水生植物对氨氮和总磷的净化效果研究[J].福建农业科技,2019(1):44-49.
[19] 狄文亮,夏霆,吉佳馨,等.刚毛藻对表流湿地净水能力及水生植物的影响[J].环境污染与防治,2018,40(10):1090-1094.
[20] HOFFMANN J P,GRAHAM L E.Effects of selected physicochemical factors on growth and zoosporogenesis of Cladophora glomerata (chlorophyta)[J].Journal of Phycology,1984,20(1):1-7.
[21] 胡帅龙,孙力平,王晨晨,等.刚毛藻除磷的最佳藻量及效果分析[J].天津城建大学学报,2020,26(1):45-49.
[22] 叶碧碧,曹德菊,张良昆,等.刚毛藻对水中N、P的控制及其环境条件研究[J].安徽农业大学学报,2011,38(4):575-578.
[23] 曾令昭.不同环境因子对漂浮刚毛藻生长、生理生化组分的影响及其生活史的初步研究[D].汕头:汕头大学,2016.
[24] 马淑娟,孙力平,钟远,等.碱度对刚毛藻生长及去除氮磷影响研究[J].工业水处理,2019,39(4):45-48.
[25] 周军.藻类膜脱氮除磷影响因素及其对磷吸附/吸收行为研究[D].南宁:广西大学,2015.
[26] 陈胜勇,李观康,汪云,等.谷氨酰胺合成酶的研究进展[J].中国农学通报,2010,26(22):45-49.
[27] 陶爽,华晓雨,王英男,等.不同氮素形态对植物生长与生理影响的研究进展[J].贵州农业科学,2017,45(12):64-68.
[28] 颜昌宙,曾阿妍,金相灿,等.不同浓度氨氮对轮叶黑藻的生理影响[J].生态学报,2007,27(3):1050-1055.
[29] 韩娜,葛荣朝,赵宝存,等.植物谷氨酰胺合成酶研究进展[J].河北师范大学学报(自然科学版),2004,28(4):407-410.
[30] 刘利群,毛定安,薄涛,等.川芎嗪对幼鼠惊厥性脑损伤脂质过氧化及抗氧化水平的影响[J].中国医院药学杂志,2008,28(4):253-256.
[31] NAIR N,BEDWAL S,PRASAD S,et al.Short-term zinc deficiency in diet induces increased oxidative stress in testes and epididymis of rats[J].Indian Journal of Experimental Biology,2005,43(9):786-794.
[32] 廖秀睿,李曦,柳睿杰,等.曲褶刚毛藻在对虾养殖尾水无机氮盐净化中的应用[J].海南热带海洋学院学报,2021,28(2):1-5.
[33] AHN O,PETRELL R J,HARRISON P J.Ammonium and nitrate uptake by Laminaria saccharina and Nereocystis luetkeana originating from a salmon sea cage farm[J].Journal of Applied Phycology,1998,10(4):333-340.