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| Identification and Drug Sensitivity Analysis of Pathogenic Plesiomonas shigelloides Isolated from Diseased Mandarin Fish Siniperca chuatsi |
| ZHANG Shuangming, JIANG Ziyan, ZHANG Zirui, TANG Huanyu, ZHU Xinhai, ZHOU Yifan, JIANG Qun, ZHANG Xiaojun, GAO Xiaojian |
| College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China |
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Abstract Outbreaks of mass mortalities occurred in mandarin fish Siniperca chuatsi farms in Yangzhou City, Jiangsu Province of China in 2019. The diseased fish had clinical symptoms of haemorrhaging on surface, gills rotting, and the presence of ascitic fluid in the abdominal cavity, enlarged gallbladder and hemorrhagic liver. The bacterial isolates from the diseased fish exhibited the same phenotypic traits and biochemical characteristics, and were identified as Plesiomonas shigelloides. In challenge test, the mandarin fish infected with the representative P. shigelloides SN1 developed similar pathological signs to the naturally diseased fish, and the LD50 value of the strain SN1 to S. chuatsi was 1.05×105 cfu/mL. Detection of virulence-associated genes by PCR indicated that P. shigelloides SN1 was positive for astA, astB, astD, astE, actP and aphA. According to the antibiotic resistant assay, P. shigelloides SN1 was sensitive to ampicillin, erythromycin and cephalosporins, and resistant to penicillin G and clindamycin. These findings will provide theoretical support for prevention and control of the diseases caused by P. shigelloides in mandarin fish.
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Received: 28 June 2021
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