企鹅珍珠贝足丝蛋白3基因的克隆及其表达分析

doi:10.16378/j.cnki.1003-1111.21120

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Abstract In order to understand the molecular mechanism of byssus adhesion of the winged pearl oyster Pteria penguin, the full-length cDNA sequence of the byssal protein 3 (BP3) gene was obtained by the rapid amplification of cDNA ends (RACE)-polymerase chain reaction (PCR) based on the transcriptional data of pearl oyster, and then real-time quantitative PCR was used to examine the expression profiles of BP3 gene in different tissues and in the feet with/ without the byssus of pearl oyster. The results showed that the full-length cDNA of BP3 gene was 601 bp, including a 5'untranslated region of 71 bp, a 3'untranslated region of 77 bp, and an open-reading frame of 453 bp, which encodes a deduced protein of 150 amino acids. The molecular weight of BP3 was 16.81 ku, and the isoelectric point was 7.33. BP3 was a hydrophilic stable extracellular protein, mainly located in the extracellular matrix. The sequence comparison showed that BP3 in the pearl oyster shares 30.34% sequence identity with BP3 in mussel Mytilus coruscus. The real-time quantitative PCR results showed that BP3 gene was expressed in the tested tissues, with the maximal relative expression level in mantle. There were significant differences among the mantle and feet, adductor muscle, pearl pocket (P<0.05), without significant difference among the mantle and gill, lip flap (P>0.05). While, the expression level in the feet with byssus was significantly higher than that in the feet without byssus (P<0.05) in pearl oyster. These findings could provide new information for further study of the molecular mechanism of byssus adhesion in pearl oyster.

Key words： Pteria penguin byssal protein 3 (BP3) gene gene clone gene expression pattern

Received: 05 July 2021

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	YANG Lei
	CHEN Yi
	ZHANG Jiayi
	ZHAN Xin

Cite this article:

YANG Lei,CHEN Yi,ZHANG Jiayi, et al. Cloning and Expression Analysis of Byssal Protein 3 Gene in Pearl Oyster Pteria penguin[J]. Fisheries Science, 2023, 42(4): 613-621.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.21120 OR https://www.shchkx.com/EN/Y2023/V42/I4/613

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