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| Effects of Acid-Base Stress on Survival Rate,Histopathology and Detoxification-Related Enzyme Activities of Mandarin Fish Siniperca chuatsi |
| ZHANG Chengshuo, ZHAO Yan, WANG Yanling, ZENG Mengdong, ZHAO Jinliang |
1. Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China;
2. Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, Shanghai Ocean University, Shanghai 201306, China |
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Abstract To investigate the effects of acid-base stress on survival rate,histopathology and detoxification related enzyme (cytochrome P450, flavin-containing monooxygenase, UDP-glucuronosyltransferase, glutathione S-transferase) activities, mandarin fish Siniperca chuatsi [body weight: large size (30.2±5.1) g and small size (5.3±0.7) g] were subjected to a 72 h static acute exposure in different pH groups (pH 4.0, 5.5, 7.0, 8.5, 10.0). The results showed that large size fish survived for 72 h in each experimental group. All small size fish died at pH 4.0 and 10.0, and the average survival time was (53.60±4.65) min and (49.30±5.34) min, respectively. After 72 h, except that the morphology of gill and liver tissue in pH 7.0 group was normal, the other experimental groups showed several abnormalities. In the pH 4.0 group, gill capillaries were broken and blood cells accumulated. In the pH 5.5 and pH 8.5 groups, hepatocytes were swollen. In the pH 10.0 group, gill epithelial cells fell off and cytoplasmic vacuolation was more obvious. The activities of cytochrome P450 and flavin-containing monooxygenase increased first and then decreased with the stress time in gill and liver tissue. The activity of glutathione S-transferase was significantly increased and higher than that in the pH 7.0 group after 72 h (P<0.05). The activity of UDP-glucuronosyltransferase was lower than that in the pH 7.0 group after 72 h in gill. Taken together, these results suggest that pH 4.0 and pH 10.0 are extremely toxic to mandarin fish fry, acid-base stress cause damage to mandarin fish, cytochrome P450, flavin-containing monooxygenase, UDP-glucuronosyltransferase and glutathione S-transferase participate in the detoxification process of mandarin fish upon acid-base stress, and work differently in time and efficiency.
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Received: 03 August 2021
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