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| Analysis of Appearance Color and Biochemical Composition in Three Types of Diseased Kelp Laminaria japonica |
| LIU Xiaohui, LIU Wei, WANG Xiangyu, LYU Fang, WU Haiyi, ZHAN Dongmei |
| Qingdao Key Laboratory of Coastal Ecological Restoration and Security, Qingdao Macroalgae Engineering Technology Research Center, Shandong Province Macroalgae Resources Conservation and Application Engineering Research Center, Marine Science Research Institute of Shandong Province, Qingdao 266104, China |
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Abstract In order to understand the variances and relationships between appearance color and biochemical composition of different diseased kelp Laminaria japonica, the differences in color indices (L*, a*, b*, BI, c*, and h*) and biochemical composition contents (moisture, chlorophyll a, carotenoid, soluble protein, alginate) were compared in three types of diseased kelp (spot white rotten kelp, green rotten kelp and twisted kelp) sampled from Rongcheng mariculture region by variance analysis and discriminant analysis. Correlation analysis between color indices and biochemical composition contents was also conducted for further exploration. The results showed that the chromatism value was ranged from 7.41 to 15.24 in the three types of diseased kelp and the color indices of L*, a*, b* and c* were significantly different (P<0.05), in which the variable coefficient of a* was the maximal with 48.18%. The green rotten kelp had the minimal a*, the maximal L*, b*, c* values, and the contrast case was observed in the twist kelp. The color indices value of spot white rotten kelp was between the green rotten kelp and twisted kelp. The biochemical components of chlorophyll a, carotenoid and soluble protein were significantly different (P<0.05) in the three types of diseased kelp, with the maximal variable coefficient of soluble protein content (22.08%). There were the maximal content of chlorophyll a and the minimal content of soluble protein in spot white rotten kelp and green rotten kelp had the minimal content of carotenoid and twist kelp the maximal content of carotenoid. The total discrimination accuracy was found to be 78.90% based on color indices and 100.00% based on biochemical composition contents in the three types of diseased kelp. The color index a* was found to be positive correlated with the contents of chlorophyll a and carotenoid in green rotten kelp samples (r=0.99, r=0.99), and there existed strong correlation between color indices of L*, b*, c* and the content of soluble protein in all the three types of diseased kelp (|r| = 0.94—0.99).
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Received: 08 October 2022
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