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| Sequencing and Analysis of Complete Mitochondrial Genome of Disk Abalone Haliotis discus hannai Cultured in Fujian Province |
| WANG Wei |
1. Fuzhou Ocean and Fisheries Technology Center, Fuzhou 350007, China;
2. Marine and Fisheries Technology Research Center, Institute of Oceanography of Fuzhou, Fuzhou 350108, China |
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Abstract In order to effectively identify abalone species and explore the sources, the complete mitochondrial genome of disk abalone Haliotis discus hannai cultured in Fujian province was sequenced by high-throughput sequencing technologies. The sequences obtained were preliminarily analyzed and the whole genome was compared with three complete or nearly-complete mitochondrial genome sequence of the disk abalone deposited in GenBank. The results showed that the disk abalone had mitogenome of 17 037 bp in total length with 37 genes identified including 13 protein coded, 22 tRNA and 2 rRNA genes. Except for the nucleotide bases differences in the control region, basically the same complete or nearly-complete mitochondrial sequences were observed in the four populations of the disk abalone. A phylogenetic tree constructed based on reported complete mitochondrial genome sequences of species in Haliotis genus revealed that all disk abalone were clustered with each other first and then clustered with H. rufescens, H. cracherodii and H. iris. The disk abalone from Lianjiang, China, had a close relationship with those from Geoje, Republic of Korea. In brief, sequencing and analysis of the entire mitochondrial genome of the abalone cultivated in Fujian province enriched the genetic information database of this species. The finding provides reference for the identification and breeding of high-quality abalone varieties in the southeastern coastal waters of China.
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Received: 11 September 2023
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[1] 陈炳能,谢开恩,卢豪魁,等.皱纹盘鲍南移的初步研究[J].动物学报,1977,23(1):30-34.
[2] 高绪生,王琦,王仁波,等.鲍鱼[M].沈阳:辽宁科学技术出版社,2000.
[3] 王进可,严正凛.鲍养殖现状及发展趋势[J].水产科学,2012,31(12):749-753.
[4] 洛轩. 西氏鲍与皱纹盘鲍杂交的遗传基础研究[D]. 厦门:厦门大学,2009.
[5] 范飞龙.绿鲍的引种及与皱纹盘鲍的种间杂交研究[D].厦门:厦门大学,2012.
[6] 房孝宁.皱纹盘鲍、黑足鲍及杂交F1代线粒体基因组测序及系统发育价值的研究[D].威海:山东大学,2016.
[7] YANG E C, NAM B H, NOH S J, et al. Complete mitochondrial genome of Pacific abalone (Haliotis discushannai) from Korea[J]. Mitochondrial DNA,2015,26(6):917-918.
[8] GUO Z S, DING Y, HAN L, et al. Characterization of the complete mitochondrial genome of Pacific abalone Haliotis discus hannai[J]. Mitochondrial DNA Part B,2019,4(1):717-718.
[9] 孙中恺,陈李品,刘艳青,等. 不同产地和季节皱纹盘鲍元素组成的聚类分析[J].中国食品学报,2020,20(11):239-246.
[10] BOLGER A M, LOHSE M, USADEL B. Trimmomatic:a flexible trimmer for Illumina sequence data[J]. Bioinformatics,2014,30(15):2114-2120.
[11] DONATH A, JüHLING F, AL-ARAB M, et al. Improved annotation of protein-coding genes boundaries in metazoan mitochondrial genomes[J]. Nucleic Acids Research,2019,47(20):10543-10552.
[12] GREINER S, LEHWARK P, BOCK R. OrganellarGenomeDRAW(OGDRAW) version 1.3.1:expanded toolkit for the graphical visualization of organellar genomes[J]. Nucleic Acids Research,2019,47(W1):W59-W64.
[13] KUMAR S, STECHER G, LI M, et al. MEGA X:molecular evolutionary genetics analysis across computing platforms[J]. Molecular Biology and Evolution,2018,35(6):1547-1549.
[14] JEX A R, HALL R S, LITTLEWOOD D T J, et al. An integrated pipeline for next-generation sequencing and annotation of mitochondrial genomes[J]. Nucleic Acids Research,2010,38(2):522-533.
[15] 张国范,常亚青,赵艳.海洋动物线粒体DNA研究进展[J].海洋科学,1997,21(1):25-28.
[16] 周传江,马爱喆,汪曦,等.鱼类线粒体基因组研究进展[J].河南师范大学学报(自然科学版),2019,47(2):74-82.
[17] MAYNARD B T, KERR L J, MCKIERNAN J M, et al. Mitochondrial DNA sequence and gene organization in the Australian blacklip abalone Haliotis rubra (leach)[J]. Marine Biotechnology,2005,7(6):645-658.
[18] VAN WORMHOUDT A, LE BRAS Y, HUCHETTE S. Haliotis marmorata from Senegal;a sister species of Haliotis tuberculata:morphological and molecular evidence[J]. Biochemical Systematics and Ecology,2009,37(6):747-755.
[19] XIN Y, REN J F, LIU X. Mitogenome of the small abalone Haliotis diversicolor Reeve and phylogenetic analysis within Gastropoda[J]. Marine Genomics,2011,4(4):253-262.
[20] ROBINSON N A, HALL N E, ROSS E M, et al. The complete mitochondrial genome of Haliotis laevigata (Gastropoda:Haliotidae) using MiSeq and HiSeq sequencing[J]. Mitochondrial DNA. Part A, DNA Mapping, Sequencing, and Analysis,2016,27(1):437-438.
[21] XU K F, KANNO M, YU H, et al. Complete mitochondrial DNA sequence and phylogenetic analysis of zhikong scallop Chlamys farreri (Bivalvia:Pectinidae)[J]. Molecular Biology Reports,2011,38(5):3067-3074.
[22] PERNA N T, KOCHER T D. Patterns of nucleotide composition at fourfold degenerate sites of animal mitochondrial genomes[J]. Journal of Molecular Evolution,1995,41(3):353-358. |
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