纳米硒对虹鳟传染性造血器官坏死病毒抗性的影响

doi:10.16378/j.cnki.1003-1111.24012

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Abstract In order to investigate the effect of dietary nano-selenium on resistance to infectious hematopoietic necrosis virus (IHNV) in rainbow trout Oncorhynchus mykiss,juvenile rainbow trout with body weight of (20±2) g were reared in a circular pond with a diameter of 1.2 m and a height of 0.6 m, with a water depth of 0.3 m, and fed the basic diet containing 0 (control), 1.0, 2.0 and 5.0 mg/kg nano-selenium, prepared by the mixture of nano selenium with water at 1∶30, and evenly spay on the surface of the basic diet, at water temperature of 17—18 ℃ for 7 days. Then, the rainbow trout was injected by 0.2 mL of IHNV solution with concentration of 1×10⁵ pfu/mL and returned to the original ponds for further feeding, and liver and tail vein blood samples were collected after anesthesia with 50 mg/L MS-222 on the 7th day in the feeding trial and 1, 2 and 3 days after challenge, respectively, and the differences in liver immunity and antioxidant-related enzyme activities, immune-related gene expression and serum antibody levels were compared between the rainbow trout groups. The results showed that there were significant increase in the activities of GSH-PX and serum TNF-α in liver of rainbow trout before infection, and addition of 1 mg/kg of nano-selenium (P＜0.05) and significant increase in the expression levels of MX-1 and IL-1β genes in the addition of 5 mg/kg of nano-selenium group(P＜0.05). The levels of serum TNF-α, C3 and IL-1β antibodies as well as hepatic CAT activity were shown to be significantly elevated on the 1 st to 3 rd day in the rainbow trout fed the diets added with nano-selenium and challenged in each group (P＜0.05), and significant reduce in the MDA content in the liver on the 3 rd day (P＜0.05). Significantly (P＜0.05) increase in hepatic GSH-PX viability, VIG-1, MX-1 and TNF-α gene expression was observed in the rainbow trout in the addition of 2 and 5 mg/kg of nano-selenium groups (P＜0.05). The findings indicated that addition of nanosized selenium to the diet led to increase the hepatic immunity and antioxidant capacity as well as the serum antibody level in rainbow trout, thus enhancing their resistance to IHNV.

Key words： Oncorhynchus mykiss infectious hematopoietic necrosis virus nano-selenium antiviral enzyme activity serum antibody

Received: 12 January 2024

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	LI Yajun
	WANG Jianfu
	HAI Qiang
	LYU Nana
	LUO Zhiyuan
	SHAN Caixia
	LI Jie
	LIU Zhe
	YU Haitao

Cite this article:

LI Yajun,WANG Jianfu,HAI Qiang, et al. Effect of Dietary Nano-Selenium on Resistance to Infectious Hematopoietic Necrosis Virus in Rainbow Trout Oncorhynchus mykiss[J]. Fisheries Science, 2025, 44(1): 47-55.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.24012 OR https://www.shchkx.com/EN/Y2025/V44/I1/47

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