激活线粒体自噬对草鱼脂肪细胞凋亡和生长发育的影响

doi:10.16378/j.cnki.1003-1111.24022

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Abstract Excessive lipid deposition in grass carp Ctenopharyngodon idella adipose tissue as one of the common and prominent problems in aquaculture production can be dealt with induction of adipocyte apoptosis.To investigate the relationship between mitophagy and apoptosis in grass carp adipocytes, 2 trails were designed and conducted in vitro and in vivo. In vitro, juvenile grass carp with body weight of some 20 g were reared in net cages and fed diets containing 0 mg/kg (control group), 10 mg/kg, 20 mg/kg, and 40 mg/kg mitophagy activatorcarbonyl cyanide-3-chlorophenylhydrazone (CCCP) at water temperature of 24—30 ℃for 8 weeks. In vitro trail, the isolated primary adipocytes of grass carpwere cultured, differentiated and matured, and then treated with CCCP at dose of 0(control group), and 20 μmol/L for 24 h.The results in the vivo assay showed that compared with the control group, the treatment of 40 mg/kg of mitophagy activator led to significantly lower grass carp intraperitoneal fat index (P<0.05), and the content of triglycerides (P<0.05), and to significantly elevated the mRNA levels of autophagy-related genes pink1, parkin, atg12, atg5 and apoptosis-related genes caspase3 and caspase9 (P<0.05), without significant change in the size of adipocyte area in each treatment group compared with the control group (P>0.05). In vivo results showed that there were significantly elevated cellular mitophagy-related genes pink1, parkin, atg4, atg7, and atg12(P<0.01), significant increase in the mRNA levels of the apoptosis-related genes bax, caspase9, and the bax/bcl-2 ratio (P<0.05 or P<0.01), and significant decrease in the mRNA of the anti-apoptosis gene bcl-2level (P<0.05), and cellular triglyceride content (P<0.05) in the fish fed the diet containing the activator. It was found that mitophagy activator treatment activated mitophagy and apoptosis. The findings indicate that reduction of intraperitoneal fat index in grass carp may be realized by inducing apoptosis of grass carp adipocytes, thus reducing lipid accumulation in grass carp. Combined in vivo and in vitro assays showed that mitophagy activator treatment activated mitophagy mediated by Pink1/Parkin.

Key words： Ctenopharyngodon idella lipid accumulation adipocyte mitophagy apoptosis PINk1/Parkin

Received: 29 January 2024

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	ZENG Caihong
	BIAN Chenchen
	SUN Jian
	JI Hong
	LI Handong
	HU Zechao

Cite this article:

ZENG Caihong,BIAN Chenchen,SUN Jian, et al. Effects of Activation of Mitophagy on Adipocyte Apoptosis and Growth and Development in Grass Carp Ctenopharyngodon idella[J]. Fisheries Science, 2025, 44(2): 171-180.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.24022 OR https://www.shchkx.com/EN/Y2025/V44/I2/171

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