扁吻鱼外周血与血细胞发生观察

doi:10.16378/j.cnki.1003-1111.25046

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Abstract To investigate the composition of blood cells and the process of hematopoiesis in fish Aspiorhynchus laticeps, and to provide insights into its ecological adaptation and supporting conservation efforts, peripheral blood smears and tissue imprints of kidney, spleen, and liver were prepared from A. laticeps,with body weight of(5.67±1.45) g, followed by Wright′s staining to analyze blood cell morphology, size, and proportions. Erythrocytes were found to be predominated in peripheral blood, and to exhibit elliptical or elongated cytoplasmic and nuclear morphology. The cytoplasm appeared to be pale red, with cell dimensions of (14.46±1.33) μm (major axis) and (8.44±0.73) μm (minor axis). Nuclei were centrally located, blue-purple in color, measuring (7.03±0.62) μm (major axis) and (3.43±0.32) μm (minor axis). Leukocytes included lymphocytes, thrombocytes, monocytes, and granulocytes, with the maximal proportion (88.09%) of lymphocytes. Hematopoiesis proceeded through primitive, immature, and mature stages, encompassing four lineages: erythrocytic, lymphocytic, monocytic, and granulocytic. Among hematopoietic organs, the kidney exhibited the maximal proportion of immature cells (30.82%), while the minimal activity (14.41%) was observed in hepatopancreas. The large erythrocyte size suggests that A. laticeps represents a phylogenetically primitive fish species. The kidney serves as the primary hematopoietic organ, with supplementary hemopoietic capacity observed in the spleen and hepatopancreas.

Key words： Aspiorhynchus laticeps peripheral blood hematopoiesis

Received: 26 February 2025

PACS:

Q959.4

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	Articles by authors
	FU Hongmei
	YANG Jie
	NIE Zhulan
	WEI Jie
	AI Tao
	XIAO Qing

Cite this article:

FU Hongmei,YANG Jie,NIE Zhulan, et al. Peripheral Blood and Hematopoiesis Observation in Aspiorhynchus laticeps[J]. Fisheries Science, 2025, 44(6): 857-867.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.25046 OR https://www.shchkx.com/EN/Y2025/V44/I6/857

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