基于微卫星标记的长春鳊优良亲本筛选研究

doi:10.16378/j.cnki.1003-1111.25058

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Abstract To screen microsatellite molecular markers related to the growth and stress resistance of white bream Parabramis pekinensis for the selection of superior broodstock, the association between stress resistance and growth was analyzed in 140 individuals of white bream with average body weight of 8.07 g collected from Heilongjiang, Jilin, and Jiangsu provinces by 12 pairs of microsatellite primers. The results showed that a total of 92 alleles was detected in the survival group (Group L), with an average number of alleles (N_a) of 7.666 7, an average effective number of alleles (N_e) of 3.615 1, an average observed heterozygosity (N_o) of 0.537 5, an average expected heterozygosity (N_e) of 0.589 8, and an average polymorphism information content (PIC) of 0.552 7. A total of 103 alleles was observed in the weak group (Group D), with an average number of alleles (N_a) of 8.583 3, an average effective number of alleles (N_e) of 4.086 7, an average observed heterozygosity (N_o) of 0.502 5, an average expected heterozygosity (N_e) of 0.589 7, and an average polymorphism information content (PIC) of 0.546 4. Ten alleles were identified as potential dominant genes for transportation stress resistance, namely CCB5F₂₁₆, CCB6F₃₃₆, CCB10F₂₇₀, CCB10F₂₇₆, CCB10F₂₈₀, CCB11F₁₇₈, CCB11F₂₀₀, CCB15F₃₅₂, and CCB2F₃₄₈, CCB3F₃₁₄ among the 12 pairs of microsatellite primers. Three genotypes were found to be positively correlated with superior growth traits: the 298/298 genotype and the 298/310 genotype in CCB3F, as well as the 270/270 genotype in CCB10F. Six broodstock individuals were successfully identified, with strong stress resistance and excellent growth traits, by combining the effects of dominant microsatellite marker genotypes and stress resistance testing

Key words： microsatellite Parabramis pekinensis resistance to transport stress growth trait

Received: 10 March 2025

PACS:

Q959.4

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	Articles by authors
	LIU Yuting
	YAN Chunmei
	JIN Xiangqin
	CHEN Weiqiang
	LIU Peng
	LANG Yukui
	ZHANG Ying
	XU Ning

Cite this article:

LIU Yuting,YAN Chunmei,JIN Xiangqin, et al. Screening of High-Quality Brooders of White Bream Parabramis pekinensis Based on Microsatellite Markers[J]. Fisheries Science, 2026, 45(1): 55-64.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.25058 OR https://www.shchkx.com/EN/Y2026/V45/I1/55

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