洄游型与陆封型马苏大麻哈鱼肌肉的DIA定量蛋白组学分析

doi:10.16378/j.cnki.1003-1111.25074

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Abstract In order to explore the adaptation mechanisms of two ecological types of masu salmon Oncorh ynchus masou, the muscle proteomes of anadromous (age 4, sexually mature females) and landlocked (age 2, sexually mature females) forms of masu salmon were comparcd by DIA based quatitative proteomics. A total of 737 differentially expressed proteins (DEPs) were identified (FC > 2, P < 0.05), including 324 upregulated and 413 downregulated proteins. GO function annotations revealed that DEPs were significantly associated with energy metabolism, lipid transport, fatty acid binding, oxidative stress regulation, and protein synthesis. In the anadromous type, proteins related to energy metabolism, oxidative stress defense, and protein biosynthesis were upregulated, suggesting enhanced energy supply, antioxidant capacity, and translational activity. Conversely, proteins involved in lipid transport and fatty acid binding were downregulated, indicating metabolic shift favoring lipid utilization in the landlocked type. KEGG pathway enrichment (starch and sucrose metabolism, purine metabolism, glycolysis/gluconeogenesis, glutathione metabolism) further highlighted the anadromous fish′s reliance on carbohydrate-based rapid energy production. Key protein changes, such as fatty acid-binding proteins, glutathione peroxidases, and mitochondrial oxidative phosphorylation components, reflect distinct genetic-metabolic regulation, antioxidant defense, and energy efficiency strategies. In summary, the anadromous ecotype exhibits muscle adaptations supporting high energy demands and environmental stress during long-distance migration and reproduction, while the landlocked ecotype demonstrates lipid metabolic adaptations to cold freshwater habitats.

Key words： Oncorhynchus masou DIA quantitative proteomics differentially expressed protein GO function annotation KEGG pathway

Received: 31 March 2025

PACS:

Q959.4

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	Articles by authors
	JIAO Siqi
	YIN Zhuang
	LI Min
	HAO Yongkun
	KONG Yidi
	QU Guijuan
	FANG Qiongya
	WANG Guiqin

Cite this article:

JIAO Siqi,YIN Zhuang,LI Min, et al. DIA Quantitative Proteomics Analysis of Muscles in Anadromous and Landlocked Forms of Masu Salmon[J]. Fisheries Science, 2026, 45(1): 36-44.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.25074 OR https://www.shchkx.com/EN/Y2026/V45/I1/36

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