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| Characteristics and Phylogenetic Analysis of Mitochondrial Genome between Chuanchia labiosa and Schizopygopsis pylzovi |
| WANG Tingkuan1, LIU Zhe2, QUAN Jinqiang2, ZHANG Mingzhou1, CHAI Chengju1, MA Lingling1, SONG Guolin2, XIE Buzhong1, LIU Zhijun3 |
1. Lanzhou Fishery Technology Extension Center, Lanzhou 730010, China;
2. College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China;
3. Yuzhong Yule Aquaculture Technology Co., Ltd., Lanzhou 730100, China |
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Abstract In order to compare the structural characteristics of mitochondrial genomes and phylogenetic relationships between Chuanchia labiosa and Schizopygopsis pylzovi, so as to provide reference basis for the molecular identification and convergent evolution of endemic fishes in the upper reaches of the Yellow River, the complete mitochondrial genomes were sequenced, assembled, annotated, and then bioinformatics analysis was carried out in C. labiosa collected from the Xinglongshan section of the Wanchuan River, a tributary of the Yellow River and S. pylzovi collected from the Yongdeng section of the Zhuanglang River, a first-level tributary of the Yellow River by Illumina sequencing technology. The results showed that C. labiosa had the complete mitochondrial genome of 16 765 bp in length, containing 22 tRNA genes, 13 protein-coding genes, 2 rRNA genes, and 1 D-Loop control region, with nucleotides A+T content of 55.51% and the G+C content of 44.49%, indicating an obvious AT bias. While S. pylzovi had the complete mitochondrial genome of 16 746 bp in length, containing 22 tRNA genes, 13 protein-coding genes, 2 rRNA genes, and 1 D-Loop control region, with nucleotides A+T content of 55.48% and the G+C content of 44.52%, also showing an obvious AT bias. The results of phylogenetic analysis indicated that the fishes in Schizothoracinae were divided into two main clades, in which Schizopygopsis pylzovi had a relatively close evolutionary relationship with Gymnocypris eckloni chilianensis, while Chuanchia labiosa clustered with Gymnocypris przewalskii, showing a relatively close genetic relationship. The findings led to the difficult problem of morphological identification of special fishes to be solved and to offer important theoretical basis and basic data for understanding of convergent evolution and biodiversity conservation of fishes in Schizothoracinae.
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Received: 08 June 2025
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