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| Growth and Probiotic Characteristics of Bacillus subtilis B2 in Sea Cucumber Apostichopus j aponicus |
| ZHANG Deqiang,GUAN Xiaoyan |
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Abstract A bacterial strain B2 isolated from sediment in healthy sea cucumber A postichopus j aponicus farm in Dalian was identified as Bacillus subtilis by morphological and 16S rDNA sequence analysis .It was found that the optimal grow th of strain B2 was observed at temperature of 15—35 ℃ ,pH of 6—8 and a sa‐linity of 30—50 .The electron microscopy revealed that there was visible gemma around strain B 2 obvious‐ly .The strain B2 produced both amylase and protease ,but did not secreate lipase .The different doses of the strain B2 was added into the diets according to the weight of sea cucumber by 0 .1% ,0 .2% and 0 .3% , and it was found that the strain B2 effectively improved the activities of digestive enzymes in the intestine and nonspecific immune enzymes in the coelomic fluids .The findings indicate that B. subtilis B2 can be used as basis strain of probiotics and added into the sea cucumber feed ,w hich can provide more resources for the further sea cucumber probiotics .
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Received: 25 March 2016
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