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| Epidemiological Investigation of Aeromonas hydrophila Infection Isolated in Major Fresh-water Fishes from Northern Regions in Henan,China |
| HE Wenxu,MAO Huili,YANG Limin,FU Yundong,ZENG Hui,GUAN Jianyi |
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Abstract To investigate the epidemiology of bacterial septicemia outbreak in fish ,Aeromonas hydrophila were isolated and identified from the northern regions of Henan Province ,China ,and the virulence and drug sensitive characteristics of pathogenic strains were verified .The diseased fish samples and water sam‐ples were collected from 4 fish cultured area in the northern regions of Henan Province .Epidemiological investigation were performed once a month ,and the distribution of A .hydrophila were detected by physi‐ological and biochemical and molecular biological methods .A total of 52 A .hydrophila strains ,32 patho‐genic strains ,20 non pathogenic strains ,were isolated and the pathogenic strains were primary distributed from July to September .Virulence experiment showed that there were different toxicities among 32 patho‐genic strains ,through which the highest virulent strain XDMG (4) were screened ,which will be as the vaccine strain for further experiments .Drug resistance indicated that differenct sensitivity to the same drug varried with different strains ,but most drug resistance was consistent .About 70% pathogenic strains were highly sensitive to Cefoperazone ,Florfenicol ,Ceftriaxone Sodium and Amikacin ,highly resistant to penicillin drugs ,with resistance rate of 100% ,and certain extent resistance to Aminoglycosides (except Amikacin) ,Sulfonamides ,and Tetracycline .The different strains showed differences in drug resistance . The findings would help enrich in epidemic data of the bacteria septicemia in this region ,and provide scien‐tific theoretical basis for human disease caused by A .hydrophila .
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Received: 25 March 2016
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