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| Effects of High Temperature Stress on Antioxidant Enzyme Activities in Sea Cucumber Apostichopus j aponicus |
| XIE Zhaowen,WANG Shoukun,LIN Xuan,CHEN Meizhen,CHEN Linliu,LIN Feng,LU Haibin |
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Abstract The activities of superoxide dismutase(SOD) ,catalase(CAT ) ,glutathione peroxidase(GSH‐PX) ,lac‐tatedehydrogenase(LDH),andalkalinephosphatase(AKP)andthecontentofmalondialdehyde(MDA)inthecoe‐lomic fluid and extracting fluid of visceral mass were determined from Lianjiang and Dalian populations of sea cu‐cumber ,Apostichopus japonicus ,exposed to different sea water temperature(15 ,18 ,21 ,24 ,27 ℃ and 30 ℃) . The results showed that the maximal activities of SOD ,CAT ,GSH‐PX and LDH of extracting fluid of visceral mass were observde at 3—9 ℃ higher in Lianjiang population than in Daliang population ;under high temperature (24 ,27 ,30 ℃) stress ,the activities of SOD ,CAT and LDH of coelomic fluid and extracting fluid of visceral mass population were very significantly higher in Lianjiang than in Daliang population (P<0 .01) .The activity of GSH‐PX of extracting fluid of visceral mass was very significantly higher in Lianjiang population than in Daliang popula‐tion(P<0 .01) ,and under 24 ℃ and 30 ℃ ,the activities of GSH‐PX of coelomic fluid population were also signifi‐cantly higher in Lianjiang population than Daliang population ( P<0 .01 );the temperature of highest activity of AKP of coelomic fluid and extracting fluid of visceral mass from the two populations were all at 15 ℃ and 18 ℃ ;the content of MDA of the coelomic fluid and extracting fluid of visceral mass was significantly lower in Lianjiang population than in Dalian population at 18—30 ℃(P<0 .05 ,P<0 .01) .The findings of high temperature stress indicated that activities of SOD ,CAT ,GSH‐PX and LDH of coelomic fluid and extracting fluid of visceral mass from the second generation of Lianjiang population of sea cucumber exposed to high temperature during summer in Fujian province ,southeast China were significantly higher than Daliang population of sea cucumber ;contents of MDA were lower significantly from Lianjiang population than Daliang population of sea cucumber .The increasing enzymes thermostability of the sea cucumber could be favorable to clean out excessive free radicals in the body un ‐der high temperature stress ,and to protect the organism from high temperature stress damage .
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Received: 25 April 2016
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