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| Comparison of Digestive Tract Structure and Distribution of Gastric Oxynticopeptic Cells among Three Mandarin Fish Siniperca Species |
| LI Chuanyang,Thammaratsuntorn Jeerawat,ZHA Danlin,ZHAO Jinliang,QIAN Yezhou,WU Chao,QIAN De |
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Abstract Structure characterization of stomach ,intestines and pyloric caecum of Mandarinfish Siniperca chuatsi , S .scherzeri and their hybrid (S .scherzeri ♀ × S .chuatsi ♂) was compared via paraffin section technique .Stom‐ach wall containing mucous ,submucosa ,lamina propria and muscular layers in mandarinfish three species shared the same structure .Relative thickness of mucous layer w as 0 .30 ± 0 .09 in S .chuatsi ,and 0 .30 ± 0 .11 in the hy‐brid ,while relative thickness of S .scherzeri was 0 .21 ± 0 .13 ,much thinner than the other two species .Intestines was found to be consisted of villus ,mucous ,submucosa ,and muscular layers ,and inner wall was comprised of many wrinkles ,and goblet cells in villus layer .There was no significant difference in wrinkle number and density of goblet cells between S .chuatsi and the hybrid(P>0 .05) ,while these data of S .scherzeri were significantly lower than S .chuatsi and the hybrid (P<0 .05) .S .chuatsi ,S .scherzeri and the hybrid had mean pyloric cae‐cum number of 213 .8 ± 17 .3 ,79 .3 ± 13 .2 and 109 .8 ± 21 .8 ,respectively .The structure of pyloric caecum was similar to intestines ,there was no significant difference in wrinkle number among three Siniperca species(P>0 .05) ,The S .scherzeri had significantly lower density of goblet cells than S .chuatsi and the hybrid did(P<0 .05) .The distribution characterization of oxynticopeptic cells was compared among the three Siniperca species via in situ hybridization technique .The deep purple hybridization signals of three pepsinogen probes (PG A1 ,A2 and C) were all detected in the gastric cells of mucous layer .Within the same species ,there was no significant difference among hybridization signal cells of PG A 1 ,A2 and C probes (P>0 .05) .Among three Siniperca spe‐cies ,density of hybridization signal cells by the gene probes (PG A1 ,A2 ,and C) was ranked from high to low as :S .chuatsi=hybrid> S .scherzeri ,without significant difference (P<0 .05) .Structure difference of digestive tract and distribution characterization of oxynticopeptic cells provided some scientific base for further study of fish diges‐tion physiology .
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Received: 25 April 2016
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