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| Genetic Diversity of 2 Economically Important Fishes in Siluriformes from Duliu River Based on mtDNA D-loop Sequences |
| LIU Wei,DAI Yinggui,YUAN Zhenxing,SUN Jijia,LIU Li |
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Abstract The composition of mtDNA D‐loop and genetic diversity were studied in 39 individuals of cat‐fishes Silurus asotus and Mystus guttatus collected in the Guizhou section of Duliu River by the methods of PCR and DNA sequencing on the basis of sequences of mtDNA D‐loop .There were 544—545 bp and 546—547 bp of D‐loop sequences near the 5′end of D‐loop ,including 58 variable loci in S .asotus and 15 variable loci in M .guttatus .The characteristic sequences were identified in the extended termination associated se‐quence and the central conserved domain and a poly‐T next to the 3′end of central conserved domain in the D‐loop sequence in each of the two species .There were 51 and 12 polymorphic loci and 11 and 8 haplotypes in the populations of S . asotus and M . guttatus , respectively .S . A sotus had haploidtype diversity of 0 .772 ,nucleotide diversity of 0 .02301 ,and average nucleotide differences of 12 .451 ,and M . guttatus had haploidtype diversity of 0 .331 ,nucleotide diversity of 0 .00131 ,and verage nucleotide differences of 0 .714 . The population of S .asotus from the Duliu River showed abundant genetic diversity ,good germplasm value and protection outlook .While the population of M . guttatus had much low genetic diversity in the Duliu River .T herefore ,it is urgent to protect and recover the genetic diversity of M . guttatus population from the Duliu River .
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Received: 25 April 2016
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