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| Isolation,Identification and Drug Sensitivity of Acinetobacter baumannii from Tortoise Mauremys nigricans |
| DING Li,LI Junyu,DAI Xiaomei,WANG Jichao,FANG Zhenhua,SHI Haitao |
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Abstract One predominant bacterial strain named DL01221 was isolated from the liver of diseased tortoise M auremys nigricans ,identified by morphologic observation ,biochemical test ,and sequencing analysis of 16S rRNA ,and then the drug sensitivity was determind by Kirby‐Bauer′s agar diffusion method .Results showed that the strain was gram negative bacterial .By analyzing the homology and phylogenetic relation‐ship of 16S rRNA sequences in the NCBI database ,the homology was found to be more than 98% com‐pared to Acinetobacter baumannii ,which was in the same group with A .baumannii .Combining with the colonial morphology and biochemical results ,the strain was identified as A .baumannii .Inoculating the strain to M . nigricans for lethality indicated that the strain had pathogenicity and the same bacterium could be isolated from these animals .Meanwhile the drug sensitivity test showed the bacterium was sensi‐tive to ceftazidime ,kanamycin and fleroxacin ,and multi‐resistant to most antibiotics .
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Received: 25 April 2016
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