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| Effect of Cy rtomium rhizome,Angelica sinensis,and Astragalus on Blood Antioxidant Parameters in Half-smooth Tongue-sole(Cynoglossus semilaevis) |
| GE Jing,GUO Yongjun,WANG Qingkui,XU Dawei,SUN Xueliang,CHEN Chengxun,XING Kezhi |
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Abstract A feeding trial was conducted to evaluate effect of Cyrtomium rhizome ,Angelicasinensis ,and Astrag‐alus on blood parameters including activities of total superoxide dismutase (T‐SOD) ,total antioxidant capacity (T‐AOC) ,and catalase (CAT ) ,and levels of malonaldehyde (MDA) ,triglyceride (TG) ,and total cholesterol (T‐CHO) in half‐smooth tongue‐sole (Cynoglossus semilaevis) .Juvenile half‐smooth tongue‐sole with body weight of (95 .45 ± 5 .29) g were reared in tanks of each 78 cm × 58 cm × 46 cm at a rate of 20 fish/tank and fed diets contai‐ning C .rhizome ,A .sinensis ,or Astragalus at different doses of 0 g/kg diet ,5 g/kg diet ,10 g/kg diet ,and 15 g/kg diet for 21 days with triplication at water temperature of (21 ± 1) ℃ and a salinity of 20 ± 1 .The activities of T‐SOD ,T‐AOC ,and CAT ,and levels of MDA ,TG ,and T‐CHO were detected in blood of the half‐smooth tongue‐sole in 7 days ,14 days and 21 days during the rearing period .It was found that the fish fed the diet supple‐mented with C .rhizome at a dose of 15 g/kg diet had significantly higher T‐SOD activity than the fish fed the con‐trol diet in 14 days ,and had significantly lower MDA content than the fish fed the control group in 14 and 21 days (P<0 .05) .The maximal T‐SOD activity was observed in the fish fed the diet supplemented with A .sinensis at a dose of 5 g/kg diet in 14 days .There were significantly lower MDA content in the fish fed the diet supplemented with A .sinensis at a rate of 10 g/kg diet than in the fish in the control group in 21 days (P<0 .05) .Diet supple‐mented with A stragalus at a dose of 5 g/kg diet resulted in significantly higher T‐SOD activity and significantly lower CAT activity in 21 days compared to the control diet (P<0 .05) .There was significantly higher T‐AOC ac‐tivity in the fish fed the diet containing different doses of Astragalus in 7 days .The findings indicated that C .rhi‐zome ,A .sinensis ,and Astragalus supplementation affected to some extent on blood antioxidant parameters in half‐smooth tongue‐sole .
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Received: 25 May 2016
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