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| Effects of Dietary Green Laver Enteromorpha proli fera on Growth,Activity of Digestive Enzymes and Immunity in Sea Cucumber Apostichopus j aponicus |
| YANG Ning,GUO Zhongshuai,WANG Zhengli |
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Abstract A 6‐week feeding experiment was conducted to evaluate the effects of dietary green laver Entero‐mopha prolifera on growth ,activities of digestive enzymes and immunity in sea cucumber Apostichopus japonicus with initial body weight of (4 .09 ± 0 .26) g .The sea cucumber juveniles were reared in 15 tanks of each 100 L in volume with recycling water and fed the diets containing 0% ,3% ,6% ,9% and 12% of green laver powder at water temperature of 17—18 ℃ and a salinity of 30 .The specific growth rate ,and activities of digestive enzymes including protease ,amylase ,cellulase ,and alginase and immune enzymes including lysozyme ,acid phosphatase ,alkaline phosphatase and superoxide dismutase were analyzed 7 , 14 ,28 and 42 days after the sea cucumber were fed different diets .The results showed that there were sig‐nificantly increase in specific grow th rate in the sea cucumber fed the diets supplemented with 6% and 9%green laver on the days 14 ,28 and 42 (P<0 .05) ,without significant differences among other groups com‐pared with control group (P>0 .05) .Compared with the control group ,there were significantly higher ac‐tivities of the digestive enzymes in the sea cucumber fed the diets containing 6% and 9% of green laver (P<0 .05) ,the maximum in the sea cucumber fed the diets containing 6% of green laver (P<0 .05) .The sea cucumber fed the diet supplemented with 6% of green laver had significantly higher activities of lysozyme , acid phosphatase and superoxide dismutase than the juveniles in other groups (P<0 .05) .It is concluded that dietary green laver leads to significant increase in specific grow th ,activities of digestive enzymes and immunity of sea cucumber ,with the optimal supplementation of 6% —9% under the present experimental conditions .
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Received: 25 May 2016
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