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| Detection of Carbohydrate Terminals in Parasite Ichthyophthirius multi filiis Trophont |
| LAI Xiaojian,XIONG Jing,ZHANG Zhe,LI Zhongqin |
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Abstract In the present study ,parasite Ichthyophthirius multi filiis invaded into skin ,gill and fin of gold fish (Crassius auratus) was sampled ,and the special binding sites of the parasitic trophont′s carbohydrate residues were pinpointed using plant lectins Con A ,WGA ,SBA and UEA‐Ⅰ .The results showed Con A and WGA immunostainings were observed in the parasite trophonts ,and Con A immunostaining was stronger than WGA .SBA and UEA‐Ⅰ immunostainings were not found in the tissues .Trophonts in the gill showed stronger Con A immunostaining than those in the skin and fin . The trophonts in the fin showed stronger WGA immunostaining than those in the skin and gill .There were mannose/glucose resi‐dues and also N‐acetylglucosamine residues in the trophonts .It is inferred that trophonts in the gill had more mannose/glucose residues than those in the skin and fin ,and trophont in the fin had more N‐acetyl‐glucosamine residues than those in the skin and gill .
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Received: 25 June 2016
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