仿刺参MyD88依赖途径基因的序列比较和表达分析

doi:10.16378/j.cnki.1003-1111.2020.01.003

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Abstract Amino acid sequences comparison of Toll, MyD88, IRAK4, TRAF6, P105 and Rel in Toll like receptor signaling pathway was performed on their conserved domains in sea cucumber Apostichopus japonicus to seek for interaction sites in order to explore the immune regulation mechanism of sea cucumber. Quantitative real-time PCR was selected to examine genes expression in coelomocytes of sea cucumber challenged with pathogen Vibrio splendidus. It was found that the expression levels of Toll were all inhibited except in significant up-regulation in 12 h challenge. MyD88 and TRAF6 showed consistent expression pattern and were up-regulated to the top point in 12 h challenge. IRAK4 was up-regulated at 12 h and recovered at 48 h. Rel and P105 were homologues of NFκB with similar domains and showed the same expression pattern. The comparisons of amino acid sequences revealed that there was a conserved TLR signaling pathway. After V. splendidus challenge, these six genes were collaboratively involved in signal transduction and immune response in sea cucumber.

Key words： sea cucumber Apostichopus japonicus TLR signaling pathway sequences comparison immune challenge gene expression

Received: 10 May 2019

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	SUN Hongjuan
	ZHENG Zhiliang
	WANG Jihui
	ZHOU Zunchun

Cite this article:

SUN Hongjuan,ZHENG Zhiliang,WANG Jihui, et al. Sequences Comparison and Expression Analysis of Genes Involvedin MyD88 Dependent Pathway in Sea Cucumber Apostichopus japonicus[J]. Fisheries Science, 2020, 39(1): 22-29.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.2020.01.003 OR https://www.shchkx.com/EN/Y2020/V39/I1/22

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