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| Effects of Nano-zinc and Nano-zinc-polysaccharide on Growth and Immunity inRainbow Trout Oncorhynchus mykiss |
| FAN Ying, LI Le, YU Xiaoqing, HU Fawen, LI Tianbao, YE Haibin, DIAO Jing |
| Key Laboratory for Disease Control in Mariculture, Marine Biology Institute of Shandong Province, Qingdao 266104, China |
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Abstract A feeding trial was conducted to evaluate the effects of nano-zinc and nano-zinc-polysaccharide on growth and immune in rainbow trout (Oncorhynchus mykiss). Three hundreds individuals of rainbow trout with body weight of (120±3.0) g were randomly allotted to 5 groups (3 replicates and each 20 fish), and injected 1000, and 3000 mg/kg nano-zinc and nano-zinc-polysaccharide according to 1 μL/g body weight and fed a basal diet supplemented with nano-zinc and its polysaccharide for 10 days. The results showed that nano-zinc and nano-zinc-polysaccharide differently promoted the special growth rate (P＜0.05). The percentage of NBT in the 1000 mg/kg nano-zinc-polysaccharide was increased by 22.3% (P<0.05) on the third day, with the maximal phagocytic percent in the 3000 mg/kg nano-zinc-polysaccharide on the sixth day. The serum bactericidal percent in the 3000 mg/kg nano-zinc was increased by 19.4% (P<0.05) on the third day, but that in the 3000 mg/kg nano-zinc-polysaccharide was increased by 32.43% (P<0.05) on the sixth day. The activities of catalase (CAT) and NOS in the serum of rainbow trout in the 1000 mg/kg nano-zinc-polysaccharide group were 26.39 U/mL and 43.38 U/mL(P<0.05) on the third day; the activities were 29.9 U/mL in SOD, 201.4 U/L in ACP, and content of 3.19 nmol/mL in MDA (P<0.05) on the sixth day. After the feeding trial, Aeromonas salraonicida injection challenged during 7 d was executed to test the effects of nano-zinc and nano-zinc-polysaccharide. There was mortality of 30% in the 1000 mg/kg nano-zinc-polysaccharide group. It is concluded that supplementation of nano-zinc and nano-zinc-polysaccharide can improve the immunity of rainbow trout, and that 1000 mg/kg nano-zinc-polysaccharide is the optimal dose.
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Received: 18 January 2018
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