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| Effects of Four Microbial Preparations on Water Quality and Postlarval Survival of Pacific White Shrimp Litopenaeus vannamei |
| HONG Juken, XUE Ming, NING Weimin, LI Chengcong, CHEN Yao, YU Wenzhi, HUANG Xuemin, LIANG Huafang, WEN Chongqing |
| Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China |
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Abstract The contents of viable bacteria of four preparations labeled as Bacillus licheniformis, B. subtilis, Enterococcus faecalis, and Lactobacillus plantarum were determined by plate counting, and the strains isolated from the preparations were identified by 16S rRNA gene sequencing analysis. Fifty thousand larvae of nauplius 3 of Pacific white shrimp Litopenaeus vannamei without specific pathogen were reared in a 250 L seawater-containing FRP barrel. Then each preparation was daily added into the larviculture water from early stage of zoea 1 to the postlarva 1 stages for 10 days at a level of 5×108 cfu/L for B. licheniformis and B. subtilis preparations, and 1×108 cfu/L for E. faecalis and L. plantarum preparations. The water quality indices and survival rate of the postlarvae were determined regularly. The results showed that the four preparations were all composed of single pure species with high concentration. There were significantly higher contents of ammonia nitrogen, inorganic phosphorus, and chemical oxygen demand in the B. licheniformis group than those in the control group, while the concentrations of nitrite nitrogen in the E. faecalis and L. plantarum groups were obviously lower than that in the control at the early stage of larviculture (P<0.05). There was no significant difference in the survival rates of postlarvae among groups (P>0.05) with the exception in the B. licheniformis group, with significantly lower than that in the B. subtilis group at postlarva 3 stage (P<0.05). However, the maximal survival rate and vitality of postlarvae were observed in B. subtilis group and the worst in B. licheniformis group. The findings indicated that under no obvious stress conditions, continuous application of microbial preparations had no significant effects in the water quality and survival rate of postlarvae as a whole. However, the effect of two kinds of Bacillus preparations was significantly different.
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Received: 24 July 2019
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