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| Effects of Aeromonas hydrophila Stimulation on Immunity of Chinese Mitten Crab Eriocheir sinensis |
| CHENG Chao1, XIAO Min2, LI Jing1, MAO Zhenfang1, ZHANG Cuizhen1, CHEN Zhihe1, ZHU Minjie1, JIAN Shaoqing1,3, ZHAO Daxian1,3 |
1. School of Life Sciences, Nanchang University, Nanchang 330031, China;
2. Ji'an City Jinggang Mountains Agricalture Science and Technology Park in Jiangxi Province, Ji'an 343000, China;
3. Jiangxi Provincal Key Laboratory of Aquatic Animal Resources and Utilization, Nanchang 330031, China |
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Abstract In this study, the influence of Aeromonas hydrophila stimulation on immune tissue structure and gene expression of Chinese mitten crab Eriocheir sinensis was analyzed by conventional paraffin section, hematoxylin-eosin staining (HE staining) and real-time fluorescence quantitative PCR.The results showed that, with the bacterial stimulation, the gill filaments thickness and the number of blood cells of Chinese mitten crab were decreased and then increased, the lumen area, structure and cell number of hepatic small tubules were changed significantly compared with the control group.The expression levels of lipopolysaccharide and beta-1, 3-glucan binding protein (LGBP) were decreased significantly at 6 h (P<0.05), but increased significantly at 12 h (P<0.05). Tube gene expression level was decreased significantly at 3 h and 6 h (P<0.05), and increased significantly at 12 h (P<0.05). Dorsal expression levels were increased significantly at 3 h (P<0.05), The expression level of integrin was significantly increased at 3 h and 6 h after infection (P<0.05), and was significantly increased at 12 h (P<0.01). The expressions of prophenoloxidase activating factor (PPAF), peroxinectin (PXN) and glutathione S-transferase (GST) were found to be decreased gradually within 12 h after infection. The expression levels of glutathione peroxidase (GPx) reached the lowest value at 6 h after infection, and then was upregulated. The expression level of superoxide dismutase (SOD) was significantly decreased at 6 h after infection (P<0.05). In conclusion, Aeromonas hydrophila stimulation significantly changed the structures of the gill and hepatopancreas and the expression levels of immune-related genes in Chinese mitten crab.
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Received: 25 June 2019
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