2017年中国部分地区白斑综合征病毒3个基因的变异分析

doi:10.16378/j.cnki.1003-1111.2020.04.013

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Abstract White spot syndrome virus (WSSV), as a high pathogenic agent, is widely prevalent in China, Southeast Asian, India and South America. In this study, 44 WSSV-positive individuals were collected from the area of Hebei province, Zhejiang province, Shandong province, Shanghai, Fujian province and Guangdong province from February to June of 2017 and DNA of the samples was extracted and specific primers for three pairs of variable regions were designed to understand the variation of the WSSV. The samples were subjected to PCR amplification of wsv-pol, wsv313, and wsv150, and the specifically amplified bands were purified using a gel recovery kit, ligated into the pMD^©18-T vector, transformed into DH5α competent cells, screened for positive clones and sequenced. The results showed that there were a total of 12 positive samples in the amplification of wsv-pol, with detection rate of 27%. The positive samples all showed a single glycine deletion at position 164, and there were also amino acid substitutions in individual samples. Amino acid alignments showed that there was no significant variation in wsv-pol. For wsv313, twelve positive samples were also found in the PCR amplification. Among the 9 positive samples, a proline was inserted at the 280th position. There were two samples which the aspartic acid was mutated to glycine at the position of 64.There were a total of 8 positive samples in the amplification of wsv150, with detection rate of some 18%. Analysis of its nucleotide sequence revealed that the positive samples were only inserted base T at position of 587. The amino acid sequence alignment showed that the positive samples all had 106 large fragment amino acid deletions posterior to the 200th position, with amino acid substitutions at positions 196, 197, 198, and 199. The amino acid sequence showed a large degree of variation, without regional differences. The findings provided the data for etiology of WSSV.

Key words： WSSV wsv-pol wsv313 wsv150 sequence analysis

Received: 11 December 2018

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	LIU Xiaoping
	CAI Miao
	LIU Qinghui
	WAN Xiaoyuan
	HUANG Jie

Cite this article:

LIU Xiaoping,CAI Miao,LIU Qinghui, et al. Variation of Three Genes in White spot syndrome virus in Some Areas of China in 2017[J]. Fisheries Science, 2020, 39(4): 560-566.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.2020.04.013 OR https://www.shchkx.com/EN/Y2020/V39/I4/560

[1]Nakano H, Koube H, Umezawa S, et al. Mass mortalities of cultured kuruma shrimp, Penaeus japonicas, in Japan in 1993: epizootiological survey and infection trials [J]. Fish Pathology,1994,29(2):135-139.
[2]Nakano T, Kodama H, Honjo T. Generation of lymphohematopoietic cells from embryonic stem cells in culture [J]. Science,1994,265(5175):1098-1101.
[3]Durand S, Lightner D V, Redman R M, et al. Ultrastructure and morphogenesis of white spot syndrome baculovirus (WSSV) [J]. Diseases of Aquatic Organisms,1997,29(3):205-211.
[4]王庚申,施慧,谢建军,等.白斑综合症病毒在日本囊对虾精养池塘中的动态变化[J]. 水产科学,2017,36(6):763-767.
[5]Marks H, Goldbach R W, Vlak J M, et al. Genetic variation among isolates of White spot syndrome virus[J]. Archives of Virology,2004,149(4):673-697.
[6]孙新颖,万晓媛,刘庆慧,等.白斑综合征病毒2014年中国毒株变异区的序列比较[J].渔业科学进展,2016,37(2):127-133.
[7]Wang Q, Poulos B T, Lightner D V. Protein analysis of geographic isolates of shrimp white spot syndrome virus[J]. Archives of Virology,2000,145(2):263-274.
[8]Balakrishnan P, Malathi S, Iddya K, et al. Characterization of variable genomic regions of Indian white spot syndrome virus[J]. Virology,2008,376(1):24-30.
[9]马晓燕,李鹏,严洁,等.对虾白斑综合症病毒的概述[J].南京师大学报:自然科学版,2012,35(4):90-100.
[10]Sánchez-Paz A. White spot syndrome virus: an overview on an emergent concern [J]. Veterinary Research,2010,41(6):43.
[11]Sarathi M, Simon M C, Ishaq Ahmed V P, et al. Silencing VP28 gene of white spot syndrome virus of shrimp by bacterially expressed dsRNA [J]. Mar Biotechnol,2008,10(2):198-206.
[12]Tsai J M, Wang H C, Leu J H, et al. Genomic and proteomic analysis of thirty-nine structural proteins of shrimp white spot syndrome virus[J]. Journal of Virology,2004,78(20):11360-11370.
[13]赖晓芳.中国对虾应答WSSV侵染相关基因的研究[D].青岛:中国海洋大学,2010.
[14]Li F, Gao M, Xu L, et al. Comparative genomic analysis of three white spot syndrome virus isolates of different virulence [J]. Virus Genes,2017,53(2):249-258.
[15]秦梦雪,刘庆慧,万晓媛,等.中国典型对虾养殖区白斑综合征病毒wsv001缺失变异分析 [J]. 动物医学进展,2017,38(1):21-27.
[16]Gottrup F. Healing of incisional wounds in stomach and duodenum: a biomechanical study [J]. American Journal of Surgery,1980,140(2):296-301.
[17]刘锟,王云杰,范萌,等.食管胃吻合口的组织学病理学功能学及临床研究 [J]. 中华胸心血管外科杂志,1994,10(1):70-72.
[18]蔡苗,刘庆慧,万晓媛,等. 2016—2017 年我国部分地区白斑综合征病毒wsv006 的变异分析[J]. 安徽农业科学,2018,46(33):75-79.