日本沼虾多态性标记筛选及群体遗传结构分析

doi:10.16378/j.cnki.1003-1111.2020.05.001

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Abstract In the present study, novel EST-linked polymorphic microsatellite markers were isolated and characterized from the transcriptome data in oriental river prawn Macrobrachium nipponense, and the genetic structure was analyzed in four populations of oriental river prawn collected from Weishanhu Lake, downstream of Changjiang River, Taihu Lake, and Dongpinghu Lake using fluorescence SSR. A total of 546 cDNA sequences containing microsatellite were selected and 60 pairs of primers were designed, in which 33 pairs of primers were amplified clearly and stably, with 12 of polymorphic pairs of primers. The number of alleles per locus were ranged from 3 to 7 in the 12 isolated polymorphic loci, with average observed and expected heterozygosity per locus ranged from 0.1724 to 0.9630 and from 0.1942 to 0.8462, respectively. The genotyping of 88 individuals (22 individuals each stock) were found to be the mean expected heterozygosity of 0.8403 and observed heterozygositiy of 0.8350 in Weishanhu Lake population, 0.8350 and 0.7854 in Dongpinghu Lake, 0.7399 and 0.8134 in Taihu Lake, and 0.7145 and 0.7909 in downstream population of Changjiang River, the higher genetic diversity of population in North China than those in South China. The analysis of molecular variance indicated that the FST value between Dongpinghu Lake population and downstream population of Changjiang River, and between Weishanhu Lake population and downstream population of Changjiang River was higher than 0.05 and lower than 0.15, showing a moderate differentiation level, with genetic distances ranging from 0.0158 to 0.0384. The UPGMA tree showed that populations of Dongpinghu Lake and Weishanhu Lake were clustered into an independent clade, then clustered with Taihu Lake population, and downstream population of Changjiang River in the end, suggesting that their genetic relationship is in accordance with regional distribution.

Key words： Macrobrachium nipponense EST-SSR fluorescence SSR geographical population genetic structure

Received: 27 March 2019

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	ZHAO Yan
	CHEN Hongju
	KONG Weiyi
	JI Xiangshan
	WANG Hui

Cite this article:

ZHAO Yan,CHEN Hongju,KONG Weiyi, et al. Screening of Polymorphic Markers and Analysis of Population Genetic Structure of Oriental River Prawn Macrobrachium nipponense[J]. Fisheries Science, 2020, 39(5): 639-648.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.2020.05.001 OR https://www.shchkx.com/EN/Y2020/V39/I5/639

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