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| Analysis of Genetic Diversity of Little Scale Mandarinfish Coreoperca whiteheadi Population Based on Cytb and D-Loop Genes |
| YU Ke1, AN Miao1, HUANG Sheng1, PAN Qiuzhi1, NIE Fushun2, HE Xiaokuan3 |
1. College of Animal Science, Guizhou University, Guiyang 550025, China;
2. Agriculture and Rural Affairs Bureau of Pingtang County, Qiannan 558300, China;
3. Agriculture and Rural Affairs Bureau of Libo, Qiannan 558400, China |
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Abstract The genetic diversity, genetic structure and population history of little scale mandarinfish (Coreoperca whiteheadi) were investigated in 86 samples collected in Tongren Jinjiang River in the upper Yuanjiang River, Pingzhou River in Pingtang County and Zhangjiang River in Libo County by mitochondrial DNA Cytb gene and D-Loop gene analysis in order to explore the diversity of wild resources of little scale mandarinfish in Guizhou Province. The sequence was found to be 1141 bp in Cytb and 822 bp in D-Loop in length. In the Cytb region, 11 haplotypes were detected and 18 haplotypes were defined, with mean haplotype diversity of 0.918, mean nucleotide difference of 3.342 and mean nucleotide diversity of 0.00293. A total of 17 mutation sites were detected in the D-Loop region, including 3 deletions and 2 insertions, and 23 haplotypes were found, with mean haplotype diversity of 0.903, mean nucleotide difference of 3.343, and mean nucleotide diversity of 0.00410. The genetic differentiation index indicated that there was significant genetic differentiation among the three populations, and both the neutral test and the nucleotide mismatch analysis revealed that little scale mandarinfish had not experienced population expansion. The findings showed that the population structure of little scale mandarinfish in Guizhou Province was primitive due to inbreeding decline. The habitat of the population in the Yangtze River Basin is ideal, with great evolutionary potential. The population in Libo Zhangjiang River is at risk, and Jinjiang River is more suitable for little scale mandarinfish breeding. In this paper, the genetic diversity of little scale mandarinfish in Guizhou Province was evaluated at molecular level, which provides reference for the protection and utilization of its germplasm resources.
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Received: 14 January 2020
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