鲟源鲁氏耶尔森菌多位点序列分型及耐药谱测定

doi:10.16378/j.cnki.1003-1111.19283

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Abstract A large number of hybrid sturgeon (Acipenser schrenckii ♀ ×A. baeri ♂) with body weight of (160±20) g died at a sturgeon farm in Liujiaxia, Gansu Province, from July to August 2019, the dead fish being redness, swelling and bleeding in the cloacal pores, and occasional bleeding from the genital pores, and occasionally bleeding from the snout. The 16S rRNA gene of 60 dominant bacterial strains was sequenced from 60 diseased fish livers. The results showed that the 60 isolates were all Yersinia ruckeris. Biotype detection and multilocus sequence typing showed that 60 strains of Y. ruckeris were all biotype 2, with six known ST types ST1, ST2, ST3, ST5, ST8 and ST13. On this basis, phylogenetic analysis of six housekeeping genes of Y. ruckeris was carried out in series, the isolates from the same source showing the differences. Ten antibiotic drugs were used to determine the general resistance of 60 strains of Y. ruckeris, and a total of 9 drug resistance were observed. The findings provided data to support the drug resistance status of Y. ruckeris in hybrid sturgeon farmed in Liujiaxia, and theoretical basis for the prevention, treatment and vaccine development of Y. ruckeris disease in sturgeon.

Key words： hybrid sturgeon Yersinia ruckeri multilocus sequence typing(MLST) resistance range

Received: 24 December 2019

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	Articles by authors
	ZHANG Xiaoli
	MA Qiangsheng
	JIAO Shizhang
	SONG Xiaoyu
	MA Xiaojun

Cite this article:

ZHANG Xiaoli,MA Qiangsheng,JIAO Shizhang, et al. Multilocus Sequence Typing and Resistance Range Determination of Yersinia ruckeri from Sturgeon Hybrid[J]. Fisheries Science, 2021, 40(3): 394-402.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.19283 OR https://www.shchkx.com/EN/Y2021/V40/I3/394

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