白斑狗鱼Dmrta1基因克隆及其表达分析

doi:10.16378/j.cnki.1003-1111.20078

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Abstract The purpose of this study was to preliminarily investigate the role of Dmrta1(doublesex-and mab-3-related transcription factor A1) gene in gonad development in northern pike Esox lucius. In this study, Dmrta1 gene of northern pike cloned by RT-PCR had sequence length of 1408 bp, encoding 433 amino acids, the predicted molecular weight of 36.047 ku, and the theoretical isoelectric point (PI) of 5.94. The gene structure and the evolutionary relationship of species were carried out using the SMART, Swiss-Model and MEGA software to be evaluated.Protein structural prediction based on the putative amino acid sequence of the Dmrta1 revealed that Dmrta1 gene of E. lucius contained a DM domain and a DMA domain, indicating that it was more phylogenetically related to Oncorhynchus mykiss and O. nerka. The homology of amino acid sequence of Dmrta1 gene in E. lucius was above 75% with O. kisutch, O. nerka, O. mykiss and Salmo salar, respectively. Real-time PCR analysis showed that Dmrta1 gene was expressed in male and female gonads, gills, meat and other tissues. The expression of Dmrta1 gene was significantly higher in testis than that in ovary (P<0.05).At the age of 180 days and 320 days, this gene was highly expressed in the gonads and gills of male E. lucius .The findings showed that the expression level of Dmrta1 gene in the testis was increased during development, indicating that it plays an important role in the maturation of testis. The Dmrta1 gene may play an important role in the formation of gills and the promotion of male sex differentiation and function maintenance.

Key words： northern pike Esox lucius Dmrta1 gene gene clone tissue expression

Received: 10 April 2020

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	LI Jing
	ZHAO Ruiyang
	TANG Lu
	ZHANG Junjie
	AIKEBAI Gulipari

Cite this article:

LI Jing,ZHAO Ruiyang,TANG Lu, et al. Molecular Cloning and Expression Analysis of Dmrta1 Gene in Northern Pike Esox lucius[J]. Fisheries Science, 2021, 40(6): 843-850.

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https://www.shchkx.com/EN/10.16378/j.cnki.1003-1111.20078 OR https://www.shchkx.com/EN/Y2021/V40/I6/843

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