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| Influence of Polysaccharide of Green Alga Chlorella pacifica on Activities of Immune-Related Enzymes in Japanese Stone Crab Charybdis japonica |
| WANG Jiaxin1, SU Hong2, WANG Weibing3, XU Yongzhong3, GUO Chenyu1, GU Xiaoduo1, WANG Hongbin1 |
1. College of Marine Life and Fisheries Sciences, Jiangsu Ocean University, Lianyungang 222005, China;
2. Xuzhou Aquarium, Xuzhou 221006, China;
3. Suqian Dajiang Feed Co., Ltd., Suqian 223900, China |
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Abstract Polysaccharides were prepared from green alga Chlorella pacifica by hot water extraction and ethanol precipitation. Activities of some immune-related enzymes including superoxide dismutase (SOD), catalase (CAT) and lysozyme (LZM) were determined in Japanese stone crab Charybdis japonica injected with 200 μL of chlorella polysaccharide solution from the third walking leg base by a sterile microsyringe at 5, 24, 48, 72, and 96 h after polysaccharide solution administration. The Japanese stone crab in control group was injected with the same amount of normal saline or without any injection to investigate influence of polysaccharide of green alga C. pacifica on activities of immune-related enzymes in Japanese stone crab. The results showed that there was the maximum activity of SOD 72 h after chlorella polysaccharide injection, 43.3% higher than that in the normal saline group and 45.2% higher than that in the uninjection group. The peak CAT activity was found in 48 h, 79.3% and 80.8% higher than those in the normal saline group and the uninjected group, respectively; the maximum activity of LZM was also shown in 48 h, 42.1% and 46.1% higher than that in the normal saline group and the uninjected group, respectively. In 72 h, the activities of SOD, CAT and LZM were all decreased significantly. The activities of CAT and LZM were all returned to the control level at 96 h, though the activities of CAT and LZM were still significantly higher than that in control group in 72 h. Immunoreactive factors in the control group with normal saline were relatively stable, slightly increased after 24 hours compared with uninjected control group, without change at other time. Significant changes in the immunoreactive factors were observed in the Japanese stone crab injected with microalgal polysaccharides within 72 hours (P<0.05), and the activities of SOD, CAT and LZM showed significant increase in different time periods, indicating that chlorella polysaccharides had a stronger effect on immune response to Japanese stone crab.
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Received: 03 April 2020
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